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TFAP2C-and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment
文献类型:期刊论文
作者 | Li, Lingjie1,2,4,5; Wang, Yong3,5,8,9; Torkelson, Jessica L.1,2,4; Shankar, Gautam1,2; Pattison, Jillian M.1,2,4; Zhen, Hanson H.1,2,4; Fang, Fengqin6; Duren, Zhana3,5,8; Xin, Jingxue3,5,8; Gaddam, Sadhana1,2,4 |
刊名 | CELL STEM CELL |
出版日期 | 2019-02-07 |
卷号 | 24期号:2页码:271-+ |
ISSN号 | 1934-5909 |
DOI | 10.1016/j.stem.2018.12.012 |
英文摘要 | Tissue development results from lineage-specific transcription factors (TFs) programming a dynamic chromatin landscape through progressive cell fate transitions. Here, we define epigenomic landscape during epidermal differentiation of human pluripotent stem cells (PSCs) and create inference networks that integrate gene expression, chromatin accessibility, and TF binding to define regulatory mechanisms during keratinocyte specification. We found two critical chromatin networks during surface ectoderm initiation and keratinocyte maturation, which are driven by TFAP2C and p63, respectively. Consistently, TFAP2C, but not p63, is sufficient to initiate surface ectoderm differentiation, and TFAP2C-initiated progenitor cells are capable of maturing into functional keratinocytes. Mechanistically, TFAP2C primes the surface ectoderm chromatin landscape and induces p63 expression and binding sites, thus allowing maturation factor p63 to positively autoregulate its own expression and close a subset of the TFAP2-Cinitiated surface ectoderm program. Our work provides a general framework to infer TF networks controlling chromatin transitions that will facilitate future regenerative medicine advances. |
资助项目 | NIH[P50-HG007735] ; NIH[R01GM109836] ; NIH[F32AR070565] ; California Institute for Regenerative Medicine tools[RT3-07796] ; National Natural Science Foundation of China[61671444] ; National Natural Science Foundation of China[61621003] ; National Natural Science Foundation of China[91730301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB13000000] ; EB Research Partnership |
WOS研究方向 | Cell Biology |
语种 | 英语 |
出版者 | CELL PRESS |
WOS记录号 | WOS:000458027300013 |
源URL | [http://ir.amss.ac.cn/handle/2S8OKBNM/32521] |
专题 | 应用数学研究所 |
通讯作者 | Oro, Anthony E. |
作者单位 | 1.Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA 2.Stanford Univ, Dept Dermatol, Sch Med, Stanford, CA 94305 USA 3.Stanford Univ, Dept Stat & Biomed Data Sci, Sch Med, Stanford, CA 94305 USA 4.Stanford Univ, Ctr Definit & Curat Med, Sch Med, Stanford, CA 94305 USA 5.Stanford Univ, Ctr Personal Dynam Regulome, Sch Med, Stanford, CA 94305 USA 6.Stanford Univ, Div Immunol & Rheumatol, Dept Med, Sch Med, Stanford, CA 94305 USA 7.Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Dept Pathol, Sch Med, Stanford, CA 94305 USA 8.Chinese Acad Sci, CEMS, NCMIS, MDIS,Acad Math & Syst Sci, Beijing 100080, Peoples R China 9.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Lingjie,Wang, Yong,Torkelson, Jessica L.,et al. TFAP2C-and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment[J]. CELL STEM CELL,2019,24(2):271-+. |
APA | Li, Lingjie.,Wang, Yong.,Torkelson, Jessica L..,Shankar, Gautam.,Pattison, Jillian M..,...&Oro, Anthony E..(2019).TFAP2C-and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment.CELL STEM CELL,24(2),271-+. |
MLA | Li, Lingjie,et al."TFAP2C-and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment".CELL STEM CELL 24.2(2019):271-+. |
入库方式: OAI收割
来源:数学与系统科学研究院
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