This study focuses on the Late Devonian ostracods and their responses to the F-F (Frasnian-Famennian) event based on abundant materials collected from the Yangdi and Nandong sections in South China. More than half (53%) of the benthic ostracod species disappeared at the F-F boundary in the Yangdi section of slope fades, whereas most benthic ostracod species survived the F-F crisis in the Nandong section of basinal facies, with the extinction of some pelagic entomozooidea. In view of a higher extinction rate (more than 65%) of ostracod species in the shallow -water Xikuangshan section, we deduce that ostracods in varied environments responded to the F-F event differently. Namely, from the carbonate platform, slope, to basinal settings, the extinction rate of benthic ostracods at the F-F transition decreased with increasing water depth. Nevertheless, the F-F event caused substantial loss among pelagic entomozooidea lineages. The extinction pattern of benthic and pelagic ostracods in F-F event in South China is similar to the situation in Europe. But the extinction rates of benthic ostracods in shallow water and slope in Europe seems to be higher than that in the same situation in South China. The abundance and diversity of ostracods decreased and some species even disappeared in anoxic environments across the F-F boundary in South China, when there were rapid changes in the palaeotemperature of surface sea water.