Cambrian carbonates with abundant fossils of agnostoid trilobites deposited on the southern slope (Jiangnan slope belt) of the Yangtze Platform and in the Jiangnan deepwater basin are well exposed in the Wangcun Section of western Hunan, South China, and in the Duibian A Section of western Zhejiang, southeastern China, respectively. To better understand the response of carbon-isotope excursions to depositional environment changes, mass extinctions and eustatic events, we collected 530 carbonate samples in fresh roadcut exposures of the two measured sections for analysis of carbon and oxygen isotopic compositions. Data of delta C-13 from the Wangcun Section, western Hunan, South China, demonstrate that the Cambrian carbon-isotope profile includes three remarkable positive excursions CPE (wc) -1, 2, 3 in the Upper Series 2, in the Lower and in the Middle Furongian Series. Three distinctive negative excursions CNE (wc) -1, 2, 3 were separately tested in the Lower Terreneuvian Series, Lower Series 3 and in the Upper Furongian Series. Similarly, in the corresponding horizons in the Duibian A Section, Zhejiang Province, southeastern China, three positive excursions CPE (db) -1, 2, 3 and three negative excursions CNE (db) -1, 2, 3 also have been discovered. We interpret these significant carbon-isotope excursions as being associated with enhanced biogenic productivity, mass extinctions and eustatic events.