The subdivision of Ladinian and Carnian strata in Guizhou, South China has been a matter of intense debate because of the paucity of age-diagnostic faunas. Here we have carried out a detailed conodont biostratigraphic investigation on the Yangliujing, Zhuganpo and Wayao formations in the Yongyue section of western Guizhou Province. Conodonts are only prolific in the Zhuganpo and Wayao formations. Three genera and twenty species are identified, including two new species Quadralella wanlanensis n. sp. and Quadralella yongyueensis n. sp. They represent a rather endemic fauna of early Carnian age. Four conodont zones are established. They are, in the ascending order, the Paragondolella foliata, Quadralella polygnathiformis, Quadralella tadpole, and Quadralella aff. praelindae zones. Thus in the study area, the Zhuganpo Formation is generally of early Carnian (Julian 1) age whilst the Wayao Formation probably extends from the Julian 2 into the late Carnian (Tuvalian substage). The Ladinian-Carnian boundary (LCB) cannot be precisely defined due to the absence of the ammonoid Daxatina canadensis and the paucity of conodonts. However, the LCB is unlikely lower than the Yangliujing-Zhuganpo formation contact. The Julian 1-Julian 2 (early Carnian) substages boundary is defined in the uppermost Zhuganpo Formation by the occurrence of basal Julian 2 ammonoid Austrotrachyceras ex gr. A. austriacum and is also evidenced by the disappearance of most short-range Julian 1 conodonts in the overlying Wayao Formation. (C) 017 Elsevier B.V. All rights reserved.