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Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro

文献类型：期刊论文

作者Wang, Di1,2; Wang, Meiyue2,3; Wang, Anhe2; Li, Jieling2; Li, Xin2,3; Jian, Honglei2; Bai, Shuo2; Yin, Jian1
刊名COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
出版日期2019-07-05
卷号572页码:266-273
关键词Collagen Microspheres Emulsification Macrophage 3D proliferation
ISSN号0927-7757
DOI10.1016/j.colsurfa.2019.04.007
英文摘要Macrophages, as an important member of immune cells, have now become a research hotspot in the field of immunotherapy, which requires prominent proliferation in vitro for clinical use. Herein, we reported the fabrication of collagen/chitosan microspheres via emulsification for 3D macrophages proliferation. With glutaraldehyde (GA) as cross-linker, the microspheres have an average diameter of 200 mu m and positively charged surfaces, which are of great stability and biocompatibility. Compared with 2D cell proliferation, the 3D microspheres could dramatically enhance macrophages proliferation rate and promote their biological functions. Furthermore, macrophages on the surface of microspheres could be easily collected by blowing and suction, without the need of trypsin digesting or the degradation of microsphere. Hence the microspheres could be reused for macrophage proliferation repeatedly. This study highlights opportunities for development of high-effective cell proliferation approach and provides a great promise in immune cell based immunotherapy.
WOS关键词TUMOR-ASSOCIATED MACROPHAGES ; STEM-CELLS ; GROWTH ; MICROCARRIERS ; POLARIZATION ; COLLAGEN ; THERAPY
资助项目National Natural Science Foundation of China[21703253] ; National Natural Science Foundation of China[21774132] ; National Natural Science Foundation of China[21877052] ; National Natural Science Foundation of China[31700706] ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province[BK20180030] ; Fundamental Research Funds for the Central Universities[JUSRP51712B] ; Max Planck Society International Partner Group Program
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000466612900029
出版者ELSEVIER SCIENCE BV
资助机构National Natural Science Foundation of China ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province ; Fundamental Research Funds for the Central Universities ; Max Planck Society International Partner Group Program
源URL[http://ir.ipe.ac.cn/handle/122111/28146]  
专题中国科学院过程工程研究所
通讯作者Bai, Shuo; Yin, Jian
作者单位1.Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Peoples R China
2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Di,Wang, Meiyue,Wang, Anhe,et al. Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2019,572:266-273.
APA Wang, Di.,Wang, Meiyue.,Wang, Anhe.,Li, Jieling.,Li, Xin.,...&Yin, Jian.(2019).Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,572,266-273.
MLA Wang, Di,et al."Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 572(2019):266-273.

入库方式： OAI收割

来源：过程工程研究所

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