中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification of a targeting-delivery peptide based on rhCNB

文献类型:期刊论文

作者Yang, JJ; Gao, YD; Zhu, ZW; Qin, NN; Wei, Q; Yang, Jinju; Gao, Yadan; Zhu, Ziwei; Qin, Nannan; Wei, Qun
刊名JOURNAL OF PEPTIDE SCIENCE
出版日期2019
卷号25期号:6
ISSN号1075-2617
关键词cellular uptake CALCINEURIN B-SUBUNIT targeting delivery INNATE IMMUNITY TLR4 PROTEIN truncated rhCNBs RECOGNITION ACTS
DOI10.1002/psc.3159
文献子类Article
英文摘要Calcineurin B subunit (CNB) is the regulatory subunit of calcineurin (CN), and its classical function is to regulate the activity of CN. Research in our laboratory has revealed that the recombinant human CNB (rhCNB) is a good antitumor candidate and can be internalized by tumor cells via TLR4 receptor complexes and targeted to tumor tissue in nude mice. However, the fragment or domain of rhCNB mediating internalization and target delivery has not been identified. To explore fragment- mediated rhCNB internalization and target delivery, we generated truncated derivatives of rhCNBs by recombinant DNA technology and examined their cellular uptake. Interactions between truncated rhCNBs and the TLR4 receptor were studied by ELISA and co-immunoprecipitation, and targeting of model tumors in nude mice was examined. The results showed that one truncated derivative, Trun3 (124-169aa), was taken up by cells and targeted tumors with almost the same efficiency as intact rhCNB. These results indicate that Trun3 (45aa) contains the major sequence responsible for rhCNB internalization and tumor targeting and might be developed for drug delivery to tumors.
WOS记录号WOS:000469399800004
源URL[http://ir.ipe.ac.cn/handle/122111/28166]  
专题中国科学院过程工程研究所
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GB/T 7714
Yang, JJ,Gao, YD,Zhu, ZW,et al. Identification of a targeting-delivery peptide based on rhCNB[J]. JOURNAL OF PEPTIDE SCIENCE,2019,25(6).
APA Yang, JJ.,Gao, YD.,Zhu, ZW.,Qin, NN.,Wei, Q.,...&Wei, Qun.(2019).Identification of a targeting-delivery peptide based on rhCNB.JOURNAL OF PEPTIDE SCIENCE,25(6).
MLA Yang, JJ,et al."Identification of a targeting-delivery peptide based on rhCNB".JOURNAL OF PEPTIDE SCIENCE 25.6(2019).

入库方式: OAI收割

来源:过程工程研究所

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