Identification of a targeting-delivery peptide based on rhCNB
文献类型:期刊论文
作者 | Yang, JJ; Gao, YD; Zhu, ZW; Qin, NN; Wei, Q; Yang, Jinju; Gao, Yadan; Zhu, Ziwei; Qin, Nannan; Wei, Qun |
刊名 | JOURNAL OF PEPTIDE SCIENCE |
出版日期 | 2019 |
卷号 | 25期号:6 |
ISSN号 | 1075-2617 |
关键词 | cellular uptake CALCINEURIN B-SUBUNIT targeting delivery INNATE IMMUNITY TLR4 PROTEIN truncated rhCNBs RECOGNITION ACTS |
DOI | 10.1002/psc.3159 |
文献子类 | Article |
英文摘要 | Calcineurin B subunit (CNB) is the regulatory subunit of calcineurin (CN), and its classical function is to regulate the activity of CN. Research in our laboratory has revealed that the recombinant human CNB (rhCNB) is a good antitumor candidate and can be internalized by tumor cells via TLR4 receptor complexes and targeted to tumor tissue in nude mice. However, the fragment or domain of rhCNB mediating internalization and target delivery has not been identified. To explore fragment- mediated rhCNB internalization and target delivery, we generated truncated derivatives of rhCNBs by recombinant DNA technology and examined their cellular uptake. Interactions between truncated rhCNBs and the TLR4 receptor were studied by ELISA and co-immunoprecipitation, and targeting of model tumors in nude mice was examined. The results showed that one truncated derivative, Trun3 (124-169aa), was taken up by cells and targeted tumors with almost the same efficiency as intact rhCNB. These results indicate that Trun3 (45aa) contains the major sequence responsible for rhCNB internalization and tumor targeting and might be developed for drug delivery to tumors. |
WOS记录号 | WOS:000469399800004 |
源URL | [http://ir.ipe.ac.cn/handle/122111/28166] |
专题 | 中国科学院过程工程研究所 |
推荐引用方式 GB/T 7714 | Yang, JJ,Gao, YD,Zhu, ZW,et al. Identification of a targeting-delivery peptide based on rhCNB[J]. JOURNAL OF PEPTIDE SCIENCE,2019,25(6). |
APA | Yang, JJ.,Gao, YD.,Zhu, ZW.,Qin, NN.,Wei, Q.,...&Wei, Qun.(2019).Identification of a targeting-delivery peptide based on rhCNB.JOURNAL OF PEPTIDE SCIENCE,25(6). |
MLA | Yang, JJ,et al."Identification of a targeting-delivery peptide based on rhCNB".JOURNAL OF PEPTIDE SCIENCE 25.6(2019). |
入库方式: OAI收割
来源:过程工程研究所
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