A biomimetic cartilage gradient hybrid scaffold for functional tissue engineering of cartilage
文献类型：期刊论文
| 作者 | Hu, Xueyan1; Li, Wenfang1; Li, Liying1; Lu, Yanguo1; Wang, Yiwei2; Parungao, Roxanne2; Zheng, Shuangshuang3,4; Liu, Tianqing1; Nie, Yi3,4; Wang, Hongfei5 |
| 刊名 | TISSUE & CELL
 |
| 出版日期 | 2019-06-01 |
| 卷号 | 58页码:84-92 |
| ISSN号 | 0040-8166 |
| 关键词 | Biomimetic gradient scaffold material Chitosan/sodium beta-glycerophosphate/gelatin Bone mesenchymal stem cells Tissue engineering |
| DOI | 10.1016/j.tice.2019.05.001 |
| 英文摘要 | Osteochondral tissue has a complex layered structure that is not self-repairing after a cartilage defect. Therefore, constructing a biomimetic gradient scaffold that meets the specific structural requirements of osteochondral tissue is a major challenge in the field of cartilage tissue engineering. In this study, chitosan/Sodium beta-glycerophosphate/Gelatin (Cs/GP/Gel) biomimetic gradient scaffolds were prepared by regulating the mass ratio of single layer raw materials. The same ratio of Cs/GP/Gel hybrid scaffold material was used as the control. Physical properties such as water absorption, porosity and the degradation rate of the material were compared to optimize the proportion of scaffold materials. P3 Bone Mesenchymal Stem Cells (BMSCs) were inoculated on the gradient and the control scaffolds to investigate its biocompatibility. Scanning electron microscopy (SEM) results show that 3:1:2, 6:1:3.5, 9:1:5, 12:1:6.5, 15:1:8 Cs/GP/Gel gradient scaffolds had excellent three-dimensional porous structures. Channels were also shown to have been interconnected, and the walls of the pores were folded. In the longitudinal dimension, gradient scaffolds had an obvious stratified structure and pore gradient gradualism, that effectively simulated the natural physiological stratified structure of real cartilage. The diameter of the pores in the control scaffold was uniform and without any pore gradient. Gradient scaffolds had good water absorption (584.24 +/- 3.79(similar to)677.47 +/- 1.70%), porosity (86.34 +/- 5.10(similar to)95.20 +/- 2.86%) and degradation (86.09 +/- 2.46 similar to 92.48 +/- 3.86%). After considering the physical properties assessed, the Cs/GP/Gel gradient scaffold with a ratio of 9:1:5 was found to be the most suitable material to support osteochondral tissue. BMSCs were subsequently inoculated on the proportional gradient and hybrid scaffolds culture. These cells survived, distributed and extended well on the gradient and hybrid scaffold material. The biomimetic gradient scaffold designed and prepared in this study provides an important foundation for the development of new gradient composite biomedical materials for osteochondral repair. |
| WOS关键词 | OSTEOCHONDRAL TISSUE ; STEM-CELLS ; FABRICATION ; REGENERATION ; ARCHITECTURE ; CONSTRUCTS ; MORPHOLOGY ; HYDROGEL |
| 资助项目 | National Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] |
| WOS研究方向 | Anatomy & Morphology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | CHURCHILL LIVINGSTONE |
| WOS记录号 | WOS:000468871600012 |
| 资助机构 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/28168]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Liu, Tianqing; Nie, Yi; Wang, Hongfei; Song, Kedong |
| 作者单位 | 1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia 3.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Henan, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 5.Dalian Med Univ, Affiliated Hosp 2, Dept Orthoped, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xueyan,Li, Wenfang,Li, Liying,et al. A biomimetic cartilage gradient hybrid scaffold for functional tissue engineering of cartilage[J]. TISSUE & CELL,2019,58:84-92. |
| APA | Hu, Xueyan.,Li, Wenfang.,Li, Liying.,Lu, Yanguo.,Wang, Yiwei.,...&Song, Kedong.(2019).A biomimetic cartilage gradient hybrid scaffold for functional tissue engineering of cartilage.TISSUE & CELL,58,84-92. |
| MLA | Hu, Xueyan,et al."A biomimetic cartilage gradient hybrid scaffold for functional tissue engineering of cartilage".TISSUE & CELL 58(2019):84-92. |
入库方式： OAI收割
来源：过程工程研究所
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