Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture
文献类型：期刊论文
| 作者 | Li, Liying1; Chen, Yongzhi1; Wang, Yiwei2; Shi, Fangxin3; Nie, Yi4,5; Liu, Tianqing1; Song, Kedong1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 128页码:184-195 |
| 关键词 | Cell encapsulation Alginate Gelatin |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2019.01.123 |
| 英文摘要 | Nowadays alginate capsules exhibit good biocompatibility and high permeability for nutrients and metabolic wastes making them appealing biomaterial for therapeutic cell encapsulation. Further study of the characteristics of alginate beads which are highly dependent on various environmental conditions to create an optimum micro environment for cells is also critical. Thus, in this study, the effect of concentration variation on the physical properties of alginate-based beads and entrapped-cells behavior was analyzed. Results showed that the increase of Ca ions concentration brought about the decrease of the average diameter, prolongation of dissolution time, reduction of permeability and swelling, and a rise of crosslinking extent and shrinkage of capsules; while raising sodium alginate concentration had an opposite effect on the diameter and shrinkage. Moreover, the addition of gelatin enhanced the penetration and swelling and slowed down the shrinkage of capsules. And MC3T3-E1 cells enclosed in the particles in which the concentration of calcium chloride, sodium alginate and gelatin was 2.5%, 2.0% and 0.5% (w/v %) had preferable abilities of proliferation and higher expression of alkaline phosphatase. Overall, the ability to tailor this system to support in vitro growth of MC3T3-E1 cells might have significance for the future use of other cell types in regenerative medicine. (C) 2019 Published by Elsevier B.V. |
| WOS关键词 | MESENCHYMAL STEM-CELLS ; MECHANICAL-PROPERTIES ; IN-VITRO ; HYDROGELS ; POLYMER ; BEADS ; DIFFERENTIATION ; ENCAPSULATION ; MICROBEADS ; EXPANSION |
| 资助项目 | National Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning Province[20180510028] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000463305100024 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning Province |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/28220]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Shi, Fangxin; Nie, Yi; Liu, Tianqing; Song, Kedong |
| 作者单位 | 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China 2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Sydney, NSW 2139, Australia 3.Dalian Med Univ, Dept Oncol, Affiliated Hosp 1, Dalian 116011, Peoples R China 4.Zhengzhou Inst Emerging Technol Ind, Zhengzhou 450000, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Liying,Chen, Yongzhi,Wang, Yiwei,et al. Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,128:184-195. |
| APA | Li, Liying.,Chen, Yongzhi.,Wang, Yiwei.,Shi, Fangxin.,Nie, Yi.,...&Song, Kedong.(2019).Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,128,184-195. |
| MLA | Li, Liying,et al."Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 128(2019):184-195. |
入库方式： OAI收割
来源：过程工程研究所
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