ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases
文献类型：期刊论文
| 作者 | Liu, Xiao-ge1,2; Lu, Shuai1; Liu, Dong-qun1,2; Zhang, Lun1,2; Zhang, Ling-xiao1,2; Yu, Xiao-lin1; Liu, Rui-tian1 |
| 刊名 | BRAIN RESEARCH
 |
| 出版日期 | 2019-03-15 |
| 卷号 | 1707页码:141-153 |
| ISSN号 | 0006-8993 |
| 关键词 | Magnetic resonance imaging Diagnosis Parkinson's disease Huntington's disease Amyloid oligomer ScFv antibody |
| DOI | 10.1016/j.brainres.2018.11.034 |
| 英文摘要 | It is widely accepted that amyloid oligomers are the most toxic species to initiate the pathologic processes of Parkinson's disease (PD) and Huntingdon's disease (HD). But there is no definitive diagnosis for PD and HD at their early stages. Here, we conjugated an amyloid oligomer-specific scFv antibody (W20) to PEGylated superparamagnetic iron oxide nanoparticles (SPIONs) and detected the properties of the SPIONs conjugated with W20. The results showed that W20-SPIONs, with the size of around 11.8 nm in diameter, were stable and nontoxic, and had enough relaxation capacity to be used as an MRI contrast agent. When applied to the transgenic mouse models of PD and HD, W20-SPIONs crossed the blood-brain barrier and specifically bound to the oligomer area to give MRI signal, distinguishing PD and HD from healthy controls. These results indicated that W20-SPIONs had potential in early-stage diagnosis for PD and HD and also opened up a new strategy for evaluating the efficacy of new drugs. |
| WOS关键词 | ANTIBODIES SPECIFICALLY RECOGNIZE ; MAGNETIC NANOPARTICLES ; MOLECULAR-MECHANISMS ; BRAIN ; OLIGOMERS ; DELIVERY ; LESIONS ; DESIGN ; MICE |
| 资助项目 | National Science and Technology Major Projects of New Drugs[2018ZX09301032] ; National Science and Technology Major Projects of New Drugs[2018ZX09733001-001-008] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA12040215] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000459842000016 |
| 资助机构 | National Science and Technology Major Projects of New Drugs ; Strategic Priority Research Program of Chinese Academy of Sciences |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/28289]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Yu, Xiao-lin; Liu, Rui-tian |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiao-ge,Lu, Shuai,Liu, Dong-qun,et al. ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases[J]. BRAIN RESEARCH,2019,1707:141-153. |
| APA | Liu, Xiao-ge.,Lu, Shuai.,Liu, Dong-qun.,Zhang, Lun.,Zhang, Ling-xiao.,...&Liu, Rui-tian.(2019).ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases.BRAIN RESEARCH,1707,141-153. |
| MLA | Liu, Xiao-ge,et al."ScFv-conjugated superparamagnetic iron oxide nanoparticles for MRI-based diagnosis in transgenic mouse models of Parkinson's and Huntington's diseases".BRAIN RESEARCH 1707(2019):141-153. |
入库方式： OAI收割
来源：过程工程研究所
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