pH-responsive Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response
文献类型：会议论文
| 作者 | Wang LY(王连艳) |
| 出版日期 | 2018-11-07 |
| 关键词 | antigen intracellular rapid release dendritic cells pH-responsive PLGA nanoparticles vaccine del |
| 英文摘要 | We fabricated pH-responsive PLGA NPs with rapid antigen intracellular release behavior in APCs.The NPs, which had thin shells and large inner space, contain ammonium bicarbonate(NH_4HCO_3) that could regulate release in endosomes and lysosomes acting as antigen release promoter in DCs was co-encapsulated with antigen(OVA).Hydrogen ions(H~+) in DC endosomes and lysosomes(pH ～5.0 and 6.5) could react with NH_4HCO_3 to generate NH_3 and CO_2, which broke NPs and released antigens.Then, the immune responses in bone marrow-derived dendritic cells(BMDCs) in vitro and in mice were examined.Antigens encapsulated in pH-responsive PLGA NPs could escape from lysosomes into the cytoplasm and be cross-presented.Mouse immunization with pH-responsive PLGA NPs induced greater lymphocyte activation, more antigen-specific CD8~+ T cells, stronger cytotoxic capacity(IFN-γ and granzyme B), enhanced antigen-specific IgG antibodies and higher serum IgG2a/IgG1.Thus, pH-responsive PLGA NPs could be potentially useful as effective vaccine delivery and adjuvant systems for the therapy of intracellular infectious diseases and virus infection. |
| 会议录 | 第十三届全国免疫学学术大会摘要汇编
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| 源URL | [http://ir.ipe.ac.cn/handle/122111/28909]  |
| 专题 | 中国科学院过程工程研究所 |
| 推荐引用方式 GB/T 7714 | Wang LY. pH-responsive Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response[C]. 见:. |
入库方式： OAI收割
来源：过程工程研究所
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