Analysis of the Antigenic Properties of Membrane Proteins of Mycobacterium tuberculosis
文献类型:期刊论文
作者 | Li, Li3,4; Liu, Haiying3,4; Zhang, Mingxia5; Chen, Xinchun2; Jin, Qi3,4; Liu, Liguo3,4; Li, Haifeng3,4; Zhang, Wei-jia1; Zhang, Xiaobing3,4; Zheng, Jianhua3,4 |
刊名 | SCIENTIFIC REPORTS |
出版日期 | 2019-02-28 |
卷号 | 9期号:1页码:10 |
ISSN号 | 2045-2322 |
DOI | 10.1038/s41598-019-39402-z |
英文摘要 | Tuberculosis (TB) is a continuing major threat to global health and a leading cause of death, particularly in developing countries. In this study, we aimed to identify a specific and sensitive diagnostic biomarker and develop a vaccine to prevent this disease. We investigated membrane proteins to reveal biomarkers in serum and peripheral blood mononuclear cells (PBMCs) obtained from TB patients. We employed Western blotting to evaluate serological immunoglobulin G levels, and Enzyme Linked Immunospot (ELISpot) to assess the antigen-specific cellular interferon-gamma secretion from PBMCs after membrane protein stimulation. A total of 219 membrane proteins were identified, 52 exhibited at a higher levels than the 38-kDa prositive control. Of these 18 exhibited reacted ratios above 1, especially Rv1111c (427-981), with a ratios at 3.38. Accuracy and sensitivity were markedly higher for the top two antigen candidates, Rv0232 and Rv1115, after two rounds of ELISpot tests than ESAT-6 in the commercial kit (42.15 and 43.62%, respectively). These two proteins were administered to mice to detect whether they acted as effective antigens in vivo. These data provide a comprehensive view of the membranes involved in humoural and cellular immune responses that may be used as biomarkers for TB and candidates for a vaccine. |
WOS关键词 | T-cell Responses ; Culture Filtrate Protein ; Fusion Protein ; Immunogenicity ; Esat-6 ; Biomarkers ; Infection ; Efficacy ; Vaccines ; Disease |
资助项目 | National Twelve-Fifth Mega-Scientific Project on the prevention and treatment of AIDS, viral hepatitis and other infectious diseases[2012ZX10003-002] ; National Twelve-Fifth Mega-Scientific Project on the prevention and treatment of AIDS, viral hepatitis and other infectious diseases[2012ZX10003-008] ; National Twelve-Fifth Mega-Scientific Project on the prevention and treatment of AIDS, viral hepatitis and other infectious diseases[2017ZX10201301-002-002] ; CAMS Initiative for Innovative Medicine[2016-I2M-1-013] ; Jin Qi team of Sanmin Project of Medicine in Shenzhen ; Sanmin Project of Medicine in Shenzhen[GCZX2015043015340574] ; Fundamental Research Funds for Central Public-interest Scientific Institution (Centre for Tuberculosis)[2017PT31010] |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000459891700052 |
资助机构 | National Twelve-Fifth Mega-Scientific Project on the prevention and treatment of AIDS, viral hepatitis and other infectious diseases ; CAMS Initiative for Innovative Medicine ; Jin Qi team of Sanmin Project of Medicine in Shenzhen ; Sanmin Project of Medicine in Shenzhen ; Fundamental Research Funds for Central Public-interest Scientific Institution (Centre for Tuberculosis) |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/6668] |
专题 | 深海科学研究部_深海生物学研究室_深海微生物细胞生物学研究组 |
通讯作者 | Liu, Haiying; Chen, Xinchun; Jin, Qi |
作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya, Hainan, Peoples R China 2.Shenzhen Univ, Dept Pathogen Biol, Sch Med, Shenzhen, Peoples R China 3.Chinese Acad Med Sci, Inst Pathogen Biol, NHC Key Lab Syst Biol Pathogens, Beijing, Peoples R China 4.Peking Union Med Coll, Beijing, Peoples R China 5.Shenzhen Third Peoples Hosp, Guangdong Key Lab Emerging Infect Dis, Shenzhen Key Lab Infect & Immun, Shenzhen, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Li,Liu, Haiying,Zhang, Mingxia,et al. Analysis of the Antigenic Properties of Membrane Proteins of Mycobacterium tuberculosis[J]. SCIENTIFIC REPORTS,2019,9(1):10. |
APA | Li, Li.,Liu, Haiying.,Zhang, Mingxia.,Chen, Xinchun.,Jin, Qi.,...&Zhu, Xiuyun.(2019).Analysis of the Antigenic Properties of Membrane Proteins of Mycobacterium tuberculosis.SCIENTIFIC REPORTS,9(1),10. |
MLA | Li, Li,et al."Analysis of the Antigenic Properties of Membrane Proteins of Mycobacterium tuberculosis".SCIENTIFIC REPORTS 9.1(2019):10. |
入库方式: OAI收割
来源:深海科学与工程研究所
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