Label-Free Sensitive Detection of Microcystin-LR via Aptamer-Conjugated Gold Nanoparticles Based on Solid-State Nanopores
文献类型：期刊论文
| 作者 | He, Feng1,2; Liang, Liyuan2 ; Zhou, Shuo2; Xie, Wanyi2; He, Shixuan2; Wang, Yunjiao2; Tlili, Chaker2; Tong, Shoufeng1; Wang, Deqiang2
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| 刊名 | LANGMUIR
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| 出版日期 | 2018-12-11 |
| 卷号 | 34期号:49页码:14825-14833 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/acs.langmuir.8b00945 |
| 通讯作者 | Liang, Liyuan(liangliyuan@cigit.ac.cn) ; Tong, Shoufeng(tsf1998@sina.com) ; Wang, Deqiang(dqwang@cigit.ac.cn) |
| 英文摘要 | A versatile and highly sensitive strategy for nanopore detection of microcystin-LR (MC-LR) is proposed herein based on the aptamer and host-guest interactions by employing a gold nanoparticle (AuNP) probe. The aptamer of MC-LR and its complementary DNA (cDNA) are respectively immobilized on AuNPs with distinct sizes (5 nm AuNPs for the aptamer and 20 nm for the cDNA), and the constructed polymeric AuNP network via the hybridization of the aptamer and cDNA was disintegrated upon the addition of MC-LR The specific interactions between the aptamer and MC-LR disrupt and release the cDNA-AuNPs that were then removed by centrifugation, leaving the MC-LR-aptamer-AuNP species in the supernatant for subsequent nanopore determination. By monitoring the current blockade of released MC-LR-aptamer-AuNPs using a specific tailored nanopore (10 and 20 nm in diameter, generated by current dielectric breakdown), we could deduce the presence of MC-LR, as the bulky NP network could not pass through a nanopore with a relatively smaller size. We realized the detection of MC-LR with a concentration as low as 0.1 nM; additionally, we have proved the specificity of the interaction between the aptamer and MC-LR by replacing MC-LR with other congener toxins (MC-RR and MC-YR), chlorophyll (a component abundantly coexists in water), and the mixture of the four. |
| 资助项目 | Key Basic Sciences and Frontier Project of Chongqing[cstc2017jcyjB0105] ; National Natural Science Foundation of China[51503207] ; National Natural Science Foundation of China[51503206] ; National Natural Science Foundation of China[41603089] ; National Natural Science Foundation of China[61471336] ; Youth Innovation Promotion Association[Y62A420 K10] ; West Light Foundation of the Chinese Academy of Sciences (CAS) ; Instrument development program of CAS[YZ201568] |
| WOS研究方向 | Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000453489200017 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/7189]  |
| 专题 | 精准医疗单分子诊断技术研究中心 |
| 通讯作者 | Liang, Liyuan; Tong, Shoufeng; Wang, Deqiang |
| 作者单位 | 1.Changchun Univ Sci & Technol, Sch Opt & Elect Engn, Changchun 130021, Jilin, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Feng,Liang, Liyuan,Zhou, Shuo,et al. Label-Free Sensitive Detection of Microcystin-LR via Aptamer-Conjugated Gold Nanoparticles Based on Solid-State Nanopores[J]. LANGMUIR,2018,34(49):14825-14833. |
| APA | He, Feng.,Liang, Liyuan.,Zhou, Shuo.,Xie, Wanyi.,He, Shixuan.,...&Wang, Deqiang.(2018).Label-Free Sensitive Detection of Microcystin-LR via Aptamer-Conjugated Gold Nanoparticles Based on Solid-State Nanopores.LANGMUIR,34(49),14825-14833. |
| MLA | He, Feng,et al."Label-Free Sensitive Detection of Microcystin-LR via Aptamer-Conjugated Gold Nanoparticles Based on Solid-State Nanopores".LANGMUIR 34.49(2018):14825-14833. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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