Toxical Effects of PM2.5 from Different Regions on Individual Cells Examined by Raman Microspectroscopy and Atomic Force Microscopy
文献类型：期刊论文
| 作者 | Wang Yan1; Tang Ming-jie2 ; Yang Zhong-bo2; Chen Li-gang2; Jia Wei-jiao2; Sun Wei-dong1; Song Zheng-xun1; Wang Hua-bin1,2,3
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| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 38期号:12页码:3758-3763 |
| 关键词 | PM2.5 Raman microspectroscopy Atomic force microscopy A549 Toxical effects |
| ISSN号 | 1000-0593 |
| DOI | 10.3964/j.issn.1000-0593(2018)12-3758-06 |
| 其他题名 | 在单细胞水平上基于拉曼光谱和原子力显微镜研究不同地区 PM2. 5 的毒理效应 |
| 英文摘要 | PM2.5 is one of the important pollutants, so it is of great significance to the study of cytotoxical effects of PM2.5 from different regions. In this study, Human lung carcinoma epithelial cells (A549) were exposed to the average daily volume of PM2.5 from three different regions in vitro for 2 h. Afterwards, the cells were examined on the single cell level by the confocal Raman microspectroscopy and atomic force microscopy, respectively, to obtain the Raman spectroscopy, and the morphological and biomechanical properties of the cells. In addition, the cytokine concentration was measured by the multiplex enzyme linked immunosorbent assay. Compared to the untreated cells, statistical results revealed several interesting phenomena, including (1) the intensity of the Raman peaks of the treated cells increased at 1 002.97, 1 127.29, 1 172.83 and 1338.1 cm(-1); (2) the lamellipodia of treated cells shrunk or partially disappeared; (3) the treated cells became higher; (4) the adhesion work decreased while the elastic energy increased for the cells treated by the PM2.5 from Suzhou region and (5) the treated cells had an inflammatory response. Our work demonstrated that the toxical effects of PM2.5 from different regions on A549 were different, which provides useful information for the air pollution control and prevention and control of lung diseases. |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000454185500018 |
| 出版者 | OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/7249]  |
| 专题 | 太赫兹技术研究中心 |
| 作者单位 | 1.Changchun Univ Sci & Technol, Coll Elect & Informat Engn, Int Res Ctr Nano Handling & Mfg China CNM, Changchun 130022, Jilin, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 3.Chinese Acad Sci, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Yan,Tang Ming-jie,Yang Zhong-bo,et al. Toxical Effects of PM2.5 from Different Regions on Individual Cells Examined by Raman Microspectroscopy and Atomic Force Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2018,38(12):3758-3763. |
| APA | Wang Yan.,Tang Ming-jie.,Yang Zhong-bo.,Chen Li-gang.,Jia Wei-jiao.,...&Wang Hua-bin.(2018).Toxical Effects of PM2.5 from Different Regions on Individual Cells Examined by Raman Microspectroscopy and Atomic Force Microscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,38(12),3758-3763. |
| MLA | Wang Yan,et al."Toxical Effects of PM2.5 from Different Regions on Individual Cells Examined by Raman Microspectroscopy and Atomic Force Microscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 38.12(2018):3758-3763. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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