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Sensitive colorimetric detection for lysozyme based on the capture of a fixed thiol-aptamer on gold nanoparticles

文献类型：期刊论文

作者Xie, Yuanyang1,2; Huang, Yu1; Tang, Dongyun1; Cui, Hongliang2; Yang, Lizhu3; Cao, Haiyan4; Yun, Wen5
刊名NEW JOURNAL OF CHEMISTRY
出版日期2019-03-21
卷号43期号:11页码:4531-4538
ISSN号1144-0546
DOI10.1039/c9nj00016j
通讯作者Huang, Yu(huangyu@cigit.ac.cn) ; Yang, Lizhu(yanglz3000@aliyun.com) ; Cao, Haiyan(513923170@qq.com)
英文摘要In this work, a novel simple and sensitive detection method for lysozyme based on the surface plasmon resonance of gold nanoparticles has been developed. The lysozyme aptamer constantly immobilized on the surface of gold nanoparticles via a thiol functional group is the recognition element of lysozyme and a stabilizer of this system. The presence of lysozyme leads to the red-shift of the resonance wavelength of gold nanoparticles in accordance with its concentration. Two factors lead to the red spectral shift of gold nanoparticles, which are the increase of the refractive index in the environment surrounding the gold nanoparticles and the aggregation of gold nanoparticles. A critical point is distinguished from the spectral shift as a function of lysozyme concentration, where the dominant power influencing the redshift is transferred from the first one to the second one with the increase of lysozyme concentration. The limit of detection for lysozyme in this study is 0.054 nM, with a linear calibration ranging from 0.347 nM to 2.431 nM under the optimal usage conditions of 0.1250 nM thiol-aptamer functionalized gold nanoparticles. Therefore, this thiol-aptamer functionalized gold nanoparticle method provides a selective detection strategy for lysozyme and generates a great promising application.
资助项目National Natural Science Foundation of China[21707137] ; Key Application and Development Program of Chongqing[cstc2017shmsA0159] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0718] ; Research Funding Project of Yangtze Normal University[2017KYQD23] ; Young Scientist Research Fund of Yangtze Normal University ; Open Research Fund Program of Key Laboratory of Reservoir Aquatic Environment of CAS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000460972700025
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.138/handle/2HOD01W0/7540]  
专题太赫兹技术研究中心
通讯作者Huang, Yu; Yang, Lizhu; Cao, Haiyan
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Zhejiang, Peoples R China
4.Yangtze Normal Univ, Sch Chem & Chem Engn, Chongqing 408100, Peoples R China
5.Chongqing Technol & Business Univ, Coll Environm & Resources, Chongqing Key Lab Catalysis & Funct Organ Mol, Chongqing 400067, Peoples R China
推荐引用方式
GB/T 7714		 Xie, Yuanyang,Huang, Yu,Tang, Dongyun,et al. Sensitive colorimetric detection for lysozyme based on the capture of a fixed thiol-aptamer on gold nanoparticles[J]. NEW JOURNAL OF CHEMISTRY,2019,43(11):4531-4538.
APA Xie, Yuanyang.,Huang, Yu.,Tang, Dongyun.,Cui, Hongliang.,Yang, Lizhu.,...&Yun, Wen.(2019).Sensitive colorimetric detection for lysozyme based on the capture of a fixed thiol-aptamer on gold nanoparticles.NEW JOURNAL OF CHEMISTRY,43(11),4531-4538.
MLA Xie, Yuanyang,et al."Sensitive colorimetric detection for lysozyme based on the capture of a fixed thiol-aptamer on gold nanoparticles".NEW JOURNAL OF CHEMISTRY 43.11(2019):4531-4538.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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