Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo
文献类型：期刊论文
| 作者 | Jia, Pan-Pan1,2; Sun, Tai1 ; Junaid, Muhammad1,2; Yang, Li1; Ma, Yan-Bo1; Cui, Zhi-Song3; Wei, Da-Peng1; Shi, Hao-Fei1; Pei, De-Sheng1
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| 刊名 | ENVIRONMENTAL POLLUTION
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| 出版日期 | 2019-04-01 |
| 卷号 | 247页码:595-606 |
| ISSN号 | 0269-7491 |
| 关键词 | Graphene Graphene oxide Cytotoxicity Anti-bacterial property Zebrafish |
| DOI | 10.1016/j.envpol.2019.01.072 |
| 通讯作者 | Pei, De-Sheng(peids@cigit.ac.cn) |
| 英文摘要 | Graphene family nanomaterials (GFNs) have attracted significant attention due to their unique characteristics and applications in the fields of biomedicine and nanotechnology. However, previous studies highlighted the in vitro and in vivo toxicity of GFNs with size and oxidation state differences are still elusive. Therefore, we prepared graphene (G) and graphene oxide (GO) of three different sizes (S-small, M-medium, and L-large), and characterized them using multiple surface-sensitive analytical techniques. In vitro assays using HEK 293T cells revealed that the small and large sizes of G and GO significantly reduced the cell viability and increased DNA damage, accompanying with activated reactive oxygen species (ROS) generation and induced various expressions of associated critical genetic markers. Moreover, the bacterial assays highlighted that G and GO caused strong acute toxicity on Tox2 bacteria. Effects of G were higher than GO and showed size dependent effect: L>M>S, while the medium size of GO induced mild genetic toxicity on RecA bacteria. In vivo assays revealed that exposure to G and GO caused the developmental toxicity, induced ROS generation, and activated related pathways (specifically GO) in zebrafish. Taken together, G showed stronger ability to decrease the survival rate and induce the acute toxicity, while GO showed obvious toxicity in terms of DNA damages, ROS generation, and abnormal gene expressions. Our findings highlighted that G and GO differentially induced toxicity based on their varying physical characteristics, especially sizes and oxidation state, and exposure concentrations and sensitivity of the employed in vitro and in vivo models. In short, this study provided deep insights on the negative effects of GFNs exposure. (C) 2019 Elsevier Ltd. All rights reserved. |
| 资助项目 | CAS Team Project of the Belt and Road ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Youth Innovation Program of Chongqing Institute of CAS[Y83A160S10] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2016jcyjA0314] ; CAS Western Light Program 2015 |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000460844800065 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/7583]  |
| 专题 | 环境与健康研究中心 |
| 通讯作者 | Pei, De-Sheng |
| 作者单位 | 1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.State Ocean Adm, Marine Ecol Res Ctr, Inst Oceanog 1, Qingdao 266061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Pan-Pan,Sun, Tai,Junaid, Muhammad,et al. Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo[J]. ENVIRONMENTAL POLLUTION,2019,247:595-606. |
| APA | Jia, Pan-Pan.,Sun, Tai.,Junaid, Muhammad.,Yang, Li.,Ma, Yan-Bo.,...&Pei, De-Sheng.(2019).Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo.ENVIRONMENTAL POLLUTION,247,595-606. |
| MLA | Jia, Pan-Pan,et al."Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo".ENVIRONMENTAL POLLUTION 247(2019):595-606. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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