Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System
文献类型：期刊论文
| 作者 | Bian, Wan-Ping ; Chen, Yan-Ling; Luo, Juan-Juan; Wang, Chao; Xie, Shao-Lin; Pei, De-Sheng
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| 刊名 | ACS SYNTHETIC BIOLOGY
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| 出版日期 | 2019-04-01 |
| 卷号 | 8期号:4页码:621-632 |
| 关键词 | mito-CRISPR/Cas9 mitochondria mtDNA editing ssDNA zebrafish |
| ISSN号 | 2161-5063 |
| DOI | 10.1021/acssynbio.8b00411 |
| 通讯作者 | Pei, De-Sheng(peids@cigit.ac.cn) |
| 英文摘要 | The mitochondria DNA (mtDNA) editing tool, zinc finger nucleases (ZFNs), transcription activator-like effector nuclease (TALENs), and clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system, is a promising approach for the treatment of mtDNA diseases by eliminating mutant mitochondrial genomes. However, there have been no reports of repairing the mutant mtDNA with homologous recombination strategy to date. Here, we show a mito-CRISPR/Cas9 system that mito-Cas9 protein can specifically target mtDNA and reduce mtDNA copy number in both human cells and zebrafish. An exogenous single-stranded DNA with short homologous arm was knocked into the targeting loci accurately, and this mutagenesis could be steadily transmitted to F-1 generation of zebrafish. Moreover, we found some major factors involved in nuclear DNA repair were upregulated significantly by the mito-CRISPR/Cas9 system. Taken together, our data suggested that the mito-CRISPR/Cas9 system could be a useful method to edit mtDNA by knock-in strategy, providing a potential therapy for the treatment of inherited mitochondrial diseases. |
| 资助项目 | CAS Team Project of the Belt and Road ; Three Hundred Leading Talents in Scientific and Technological Innovation Program of Chongqing[CSTCCXLJRC201714] ; Program of China -Sri Lanka Joint Research and Demonstration Center for Water Technology |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000465487100002 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/7662]  |
| 专题 | 环境与健康研究中心 |
| 通讯作者 | Pei, De-Sheng |
| 作者单位 | Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bian, Wan-Ping,Chen, Yan-Ling,Luo, Juan-Juan,et al. Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System[J]. ACS SYNTHETIC BIOLOGY,2019,8(4):621-632. |
| APA | Bian, Wan-Ping,Chen, Yan-Ling,Luo, Juan-Juan,Wang, Chao,Xie, Shao-Lin,&Pei, De-Sheng.(2019).Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System.ACS SYNTHETIC BIOLOGY,8(4),621-632. |
| MLA | Bian, Wan-Ping,et al."Knock-In Strategy for Editing Human and Zebrafish Mitochondrial DNA Using Mito-CRISPR/Cas9 System".ACS SYNTHETIC BIOLOGY 8.4(2019):621-632. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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