Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh
文献类型：期刊论文
| 作者 | Yan, Han1,2; Zhou, Daming2 ; Shi, Biao2; Zhang, Ziyin2; Tian, Haibing2; Yu, Leyong2; Wang, Yunjiao2; Guan, Xiyun3; Wang, Zuobin1; Wang, Deqiang2
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| 刊名 | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 48期号:3页码:261-266 |
| 关键词 | Nanopore Dwell time Salt concentration gradient Nanofiber mesh |
| ISSN号 | 0175-7571 |
| DOI | 10.1007/s00249-019-01350-x |
| 通讯作者 | Wang, Zuobin(wangz@cust.edu.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn) |
| 英文摘要 | Solid-state nanopores are considered an attractive basis for single-molecule DNA sequencing. At present, one obstacle to be overcome is the improvement of their temporal resolution, with the DNA molecules remaining in the sensing volume of the nanopore for a long period of time. Here, we used a composite system of a concentration gradient of LiCl in solution and a nanofiber mesh to slow the DNA perforation speed. Compared to different alkali metal solutions with the same concentration, LiCl can extend the dwell time to 20 ms, five times longer than NaCl and KCl. Moreover, as the concentration gradient increases, the dwell time can be tuned from dozens of milliseconds to more than 100 ms. When we introduce a nanofiber mesh layer on top of the pore in the asymmetric solution, the DNA molecules get retarded by 162-185 s/nt, which is three orders of magnitude slower than the bare nanopore. At the same time, because the molecule absorption region becomes larger at the pore vicinity, the higher molecule capture rate improves the detection efficiency. |
| 资助项目 | National Natural Science Foundations of China[NNSFC 61701474] ; National Natural Science Foundations of China[61471336] ; National Natural Science Foundations of China[31501084] ; National Natural Science Foundations of China[51503206] ; Chongqing Sci. & Technol. Commission[cstc2017jcyjAX0320] ; West Light Foundation of CAS |
| WOS研究方向 | Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000463833800004 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/7734]  |
| 专题 | 精准医疗单分子诊断技术研究中心 |
| 通讯作者 | Wang, Zuobin; Wang, Deqiang |
| 作者单位 | 1.Changchun Univ Sci & Technol, Changchun 130022, Jilin, Peoples R China 2.Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China 3.Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA |
| 推荐引用方式 GB/T 7714 | Yan, Han,Zhou, Daming,Shi, Biao,et al. Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2019,48(3):261-266. |
| APA | Yan, Han.,Zhou, Daming.,Shi, Biao.,Zhang, Ziyin.,Tian, Haibing.,...&Wang, Deqiang.(2019).Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,48(3),261-266. |
| MLA | Yan, Han,et al."Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 48.3(2019):261-266. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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