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Revealing the Effects of Curcumin on SH-SY5Y Neuronal Cells: A Combined Study from Cellular Viability, Morphology, and Biomechanics

文献类型：期刊论文

作者Yu, Xiaoting1,2,3; Chen, Ligang2,3,4; Tang, Mingjie2,3; Yang, Zhongbo2,3; Fu, Ailing4; Wang, Zhanzhong1; Wang, Huabin1,2,3
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2019-04-17
卷号67期号:15页码:4273-4279
ISSN号0021-8561
关键词curcumin neuronal cells viability morphology biomechanics
DOI10.1021/acs.jafc.9b00314
通讯作者Wang, Zhanzhong(wzz7698@tju.edu.cn) ; Wang, Huabin(wanghuabin@cigit.ac.cn)
英文摘要In this work, the effects of curcumin on the viability, morphology, and nanomechanics of SH-SY5Y neuronal cells were investigated using a conventional cell viability test kit (CCK-8) and sophisticated AFM imaging and force measurement techniques. CCK-8 tests show that SH-SY5Y neuronal cells have a dose-response to curcumin in terms of viability that is dependent on the exposure durations. When exposed to a maximum dosage of 32 mu g/mL used in the present study for 4 h, 24 h, and 48 h, the cell viability dropped to 73.4 +/- 4.5%, 9.1 +/- 3.2%, and 2.5 +/- 1.2% of the control, correspondingly. AFM studies show that curcumin can induce the disappearance of synapses of the cells and the change of biomechanics. After exposure for 24 h at the concentration of 16 mu g/mL, the viscous deformation of the cells decreased from 2.15 +/- 0.02 to 1.58 +/- 0.03 (x10(-15) N.m), the elastic deformation increased from 1.26 +/- 0.04 to 1.72 +/- 0.07 (x10(-15) N.m), and adhesion work decreased from 0.56 +/- 0.07 to 0.39 +/- 0.04 (x10(-16) N.m). The morphological and mechanical changes obtained using AFM can be interpreted from optically observed cellular structural changes. The present study provides insights into the effects of curcumin on neuronal cells from both biological and biophysical aspects, which can help more comprehensively understand the interactions between curcumin and SH-SY5Y cells. The demonstrated techniques can be potentially used to assess the efficacy of bioactive constituents on cells or help screen drugs.
资助项目National Key Research and Development Program of China[2016YFC0101002] ; National Key Research and Development Program of China[2017YFF0106303] ; National Key Research and Development Program of China[2016YFC0101301] ; Central Government Supported Key Instrument Program of China[YXGYQ201700136] ; National Natural Science Foundation for Young Scientists of China[11604332] ; Light of West China Program of Chinese Academy of Sciences[R52A500Z10] ; Fundamental & Advanced Research Project of Chongqing[cstc2018jcyjAX0405] ; Chongqing Collaborative Innovation Center for Brain Science
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000465189400016
源URL[http://119.78.100.138/handle/2HOD01W0/7749]  
专题太赫兹技术研究中心
通讯作者Wang, Zhanzhong; Wang, Huabin
作者单位1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Chongqing Engn Res Ctr High Resolut & 3 Dimens Dy, Chongqing 400714, Peoples R China
3.Chinese Acad Sci, Ctr Appl Phys, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
4.Southwest Univ, Sch Pharmaceut Sci, Chongqing 400716, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Xiaoting,Chen, Ligang,Tang, Mingjie,et al. Revealing the Effects of Curcumin on SH-SY5Y Neuronal Cells: A Combined Study from Cellular Viability, Morphology, and Biomechanics[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(15):4273-4279.
APA Yu, Xiaoting.,Chen, Ligang.,Tang, Mingjie.,Yang, Zhongbo.,Fu, Ailing.,...&Wang, Huabin.(2019).Revealing the Effects of Curcumin on SH-SY5Y Neuronal Cells: A Combined Study from Cellular Viability, Morphology, and Biomechanics.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(15),4273-4279.
MLA Yu, Xiaoting,et al."Revealing the Effects of Curcumin on SH-SY5Y Neuronal Cells: A Combined Study from Cellular Viability, Morphology, and Biomechanics".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.15(2019):4273-4279.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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