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Imaging brain tissue slices with terahertz near-field microscopy

文献类型：期刊论文

作者Geng, Guoshuai1,2,3; Dai, Guangbin1,2,3; Li, Dandan2,3,4; Zhou, Shengling5; Li, Zaoxia2,3,6,7; Yang, Zhongbo2,3; Xu, Yuehong8; Han, Jiaguang2,3,8; Chang, Tianying1,2,3; Cui, Hong-Liang1,2,3
刊名BIOTECHNOLOGY PROGRESS
出版日期2019-03-01
卷号35期号:2页码:6
关键词terahertz near-field brain tissue imaging
ISSN号8756-7938
DOI10.1002/btpr.2741
通讯作者Cui, Hong-Liang(hcui@jlu.edu.cn) ; Wang, Huabin(wanghuabin@cigit.ac.cn)
英文摘要Photoconductive antenna microprobe (PCAM)-based terahertz (THz) near-field imaging technique is promising for biomedical detection due to its excellent biocompatibility and high resolution; yet it is limited by its imaging speed and the difficulty in the control of the PCAM tip-sample separation. In this work, we successfully realized imaging of mouse brain tissue slices using an improved home-built PCAM-based THz near-field microscope. In this system, the imaging speed was enhanced by designing and applying a voice coil motor-based delay-line. The tip-sample separation control was implemented by developing an image analysis-based technique. Compared with conventional PCAM-based THz near-field systems, our improved system is 100 times faster in imaging speed and the tip-sample separation can be controlled to a few micrometers (e.g., 3m), satisfying the requirements of THz near-field imaging of biological samples. It took about similar to 30 min (not the tens of hours it took to acquire the same kind of image previously) to collect a THz near-field image of brain tissue slices of BALb/c mice (500 mx500 m) with pixel size of 20 mx20 m. The results show that the mouse brain slices can be properly imaged and different regions in the slices (i.e., the corpus callosum region and the cerebrum region) can be identified unambiguously. Evidently, the work demonstrated here provides not only a convincing example but a useful technique for imaging biological samples with THz near-field microscopy. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2741, 2019.
资助项目National Key Research and Development Program of China[2016YFC0101002] ; National Key Research and Development Program of China[2017YFF0106303] ; National Key Research and Development Program of China[2016YFC0101301] ; Central Government Supported Key Instrument Program of China[YXGYQ201700136] ; National Natural Science Foundation for Young Scientists of China[11604332] ; Key Scientific Instrument and Equipment Development Program of Chinese Academy of Sciences ; Light of West China Program of Chinese Academy of Sciences[R52A500Z10] ; Fundamental & Advanced Research Project of Chongqing[cstc2015jcyjA10057] ; Application Development Project of Chongqing[Y500061LH1]
WOS研究方向Biotechnology & Applied Microbiology ; Food Science & Technology
语种英语
WOS记录号WOS:000467996300003
出版者WILEY
源URL[http://119.78.100.138/handle/2HOD01W0/7916]  
专题太赫兹技术研究中心
通讯作者Cui, Hong-Liang; Wang, Huabin
作者单位1.Jilin Univ, Coll Instrumentat & Elect Engn, Changchun 130061, Jilin, Peoples R China
2.Chinese Acad Sci, Ctr Appl Phys, Coll Mat Sci & Optoelect Technol, Chongqing 400714, Peoples R China
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing 400714, Peoples R China
4.Chongqing Univ, Bioengn Coll, Chongqing 400044, Peoples R China
5.Southwest Univ, Coll Engn & Technol, Chongqing 400714, Peoples R China
6.Changchun Univ Sci & Technol, Sch Optoelect Engn, Changchun 130022, Jilin, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
8.Tianjin Univ, Sch Precis Instrument & Optoelect Engn, Tianjin 300072, Peoples R China
推荐引用方式
GB/T 7714		 Geng, Guoshuai,Dai, Guangbin,Li, Dandan,et al. Imaging brain tissue slices with terahertz near-field microscopy[J]. BIOTECHNOLOGY PROGRESS,2019,35(2):6.
APA Geng, Guoshuai.,Dai, Guangbin.,Li, Dandan.,Zhou, Shengling.,Li, Zaoxia.,...&Wang, Huabin.(2019).Imaging brain tissue slices with terahertz near-field microscopy.BIOTECHNOLOGY PROGRESS,35(2),6.
MLA Geng, Guoshuai,et al."Imaging brain tissue slices with terahertz near-field microscopy".BIOTECHNOLOGY PROGRESS 35.2(2019):6.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

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