One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Shi, Junyou1,2; Li, Guoliang1,2; Wang, Honglun1; Zheng, Jie1,2; Suo, Yourui1; You, Jinmao1; Liu, Yongjun1 |
| 刊名 | PHYTOCHEMICAL ANALYSIS
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 22期号:5页码:450-454 |
| 关键词 | High-speed Counter-current Chromatography Poacynum Hendersonii Isoquercitrin Quercetin-3-o-sophoroside Quercetin-3-o-(6 ''-o-malonyl)-beta-d-glucoside |
| ISSN号 | 0958-0344 |
| 文献子类 | Article |
| 英文摘要 | Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. Objective - To develop an efficient method for the preparative isolation and purification of flavonoids from the leaves of Poacynum hendersonii by high-speed counter-current chromatography (HSCCC). Methodology - Powdered Poacynum hendersonii lead was extracted three times with 75% ethanol at 60 degrees C for 3 h. The distribution constant (K(D)) was measured to select an optimal two-phase solvent system for HSCCC separation. The purities of the target compounds were tested using HPLC and their structures were identified by (1)H-NMR and (13)C-NMR. Results - Using a two-phase solvent system composed of n-butanol-petroleum ether-0.5% acetic acid (5:3:5, v/v), three main flavonoids, i.e. isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside, were separated from 240 mg crude sample in a one-step separation by using HSCCC method. After further purification with a Sepdex-LH20 column, 5.7 mg isoquercitrin (LC purity 98.72%), 4.9 mg quercetin-3-O-sophoroside (LC purity 99.06%) and 7.4 mg quercetin-3-O-(6 ''-O-malonyl)-beta-D-glucoside (LC purity 99.31%) were obtained, respectively. Conclusion - The optimised high-speed counter-current chromatography method is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii. Copyright (C) 2011 John Wiley & Sons, Ltd.; Introduction - Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. |
| WOS关键词 | APOCYNUM-VENETUM LEAVES ; AQUEOUS EXTRACTS ; NELUMBO-NUCIFERA ; PURIFICATION ; RATS ; CONSTITUENTS ; L. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000294443500009 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1512]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Junyou,Li, Guoliang,Wang, Honglun,et al. One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2011,22(5):450-454. |
| APA | Shi, Junyou.,Li, Guoliang.,Wang, Honglun.,Zheng, Jie.,Suo, Yourui.,...&Liu, Yongjun.(2011).One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,22(5),450-454. |
| MLA | Shi, Junyou,et al."One-step Separation of Three Flavonoids from Poacynum hendersonii by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 22.5(2011):450-454. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。