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Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization

文献类型：期刊论文

作者Dou, Quan-Wen1; Zhang, Tong-Lin2; Tsujimoto, Hisashi3
刊名JOURNAL OF SYSTEMATICS AND EVOLUTION
出版日期2011-07-01
卷号49期号:4页码:347-352
关键词Elymus Fluorescence In Situ Hybridization Repetitive Sequences
ISSN号1674-4918
文献子类Article
英文摘要Genome constitution and genetic relationships between six Elymus species were assessed by physical mapping of different repetitive sequences using a technique of sequential fluorescence in situ hybridization and genomic in situ hybridization. The six Elymus species are all naturally growing species in northwest China, namely, E. sibiricus, E. nutans, E. barystachyus, E. xiningensis, E. excelsus, and E. dahuricus. An StStHH genome constitution was revealed for E. sibiricus and StStHHYY for the remainder species. Each chromosome could be clearly characterized by physical mapping with 18S-26S rDNA, 5S rDNA, Afa-family, and AAG repeats, and be allocated to a certain genome by genomic in situ hybridization. Two 5S rDNA sites, each in the H and St genomes, and three 18S-26S rDNA sites, two in the St genome and one in the Y genome, were uncovered in most of the species. The strong Afa-family hybridization signals discriminated the H genome from the St and Y genomes. The H and Y genome carried more AAG repeats than St. A common non-Robertsonian reciprocal translocation between the H and Y genomes was revealed in E. barystachyus, E. xiningensis, E. excelsus and E. dahuricus. Comparison of molecular karyotypes strongly suggests that they can be classified into three groups, namely, E. sibiricus, E. nutans, and others.; Genome constitution and genetic relationships between six Elymus species were assessed by physical mapping of different repetitive sequences using a technique of sequential fluorescence in situ hybridization and genomic in situ hybridization. The six Elymus species are all naturally growing species in northwest China, namely, E. sibiricus, E. nutans, E. barystachyus, E. xiningensis, E. excelsus, and E. dahuricus. An StStHH genome constitution was revealed for E. sibiricus and StStHHYY for the remainder species. Each chromosome could be clearly characterized by physical mapping with 18S-26S rDNA, 5S rDNA, Afa-family, and AAG repeats, and be allocated to a certain genome by genomic in situ hybridization. Two 5S rDNA sites, each in the H and St genomes, and three 18S-26S rDNA sites, two in the St genome and one in the Y genome, were uncovered in most of the species. The strong Afa-family hybridization signals discriminated the H genome from the St and Y genomes. The H and Y genome carried more AAG repeats than St. A common non-Robertsonian reciprocal translocation between the H and Y genomes was revealed in E. barystachyus, E. xiningensis, E. excelsus and E. dahuricus. Comparison of molecular karyotypes strongly suggests that they can be classified into three groups, namely, E. sibiricus, E. nutans, and others.
WOS关键词RIBOSOMAL-RNA GENES ; AFA-FAMILY ; BARLEY CHROMOSOMES ; TRITICEAE ; POACEAE ; WHEAT ; IDENTIFICATION ; NUTANS ; LOCI ; FISH
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000293273200005
公开日期2011-12-13
源URL[http://ir.nwipb.ac.cn//handle/363003/1532]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810008, Peoples R China
2.Jiangxi Inst Educ, Nanchang 330029, Peoples R China
3.Tottori Univ, Fac Agr, Tottori 6808550, Japan
推荐引用方式
GB/T 7714		 Dou, Quan-Wen,Zhang, Tong-Lin,Tsujimoto, Hisashi. Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(4):347-352.
APA Dou, Quan-Wen,Zhang, Tong-Lin,&Tsujimoto, Hisashi.(2011).Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization.JOURNAL OF SYSTEMATICS AND EVOLUTION,49(4),347-352.
MLA Dou, Quan-Wen,et al."Physical mapping of repetitive sequences and genome analysis in six Elymus species by in situ hybridization".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.4(2011):347-352.

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来源：西北高原生物研究所

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