HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L
文献类型：期刊论文
| 作者 | Suo, Yourui1; Song, Cuihua3; Sun, Zhiwei1,2; Xia, Lian1,2,3; You, Jinmao1,3 |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:6页码:859-874 |
| 关键词 | 2-(2-(Pyren-1-yl)-1h-benzo[d]Imidazol-1-yl) Ethyl-p-toluenesulfonate (Pbiots) Atmospheric Pressure Chemical Ionization Mass Spectrometer (Apci-ms) Fatty Acids (Fas) Fluorescence Detection (Fld) High Performance Liquid Chromatography (Hplc) Stellera Chamaejasma l |
| ISSN号 | 1082-6076 |
| 文献子类 | Article |
| 英文摘要 | A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined.; A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRIC IDENTIFICATION ; FLUORESCENCE DETECTION ; CARBOXYLIC-ACIDS ; MS ; REAGENT ; PLANTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000277574500013 |
| 公开日期 | 2011-12-13 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1782]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, Yourui,Song, Cuihua,Sun, Zhiwei,et al. HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):859-874. |
| APA | Suo, Yourui,Song, Cuihua,Sun, Zhiwei,Xia, Lian,&You, Jinmao.(2010).HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(6),859-874. |
| MLA | Suo, Yourui,et al."HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.6(2010):859-874. |
入库方式： OAI收割
来源：西北高原生物研究所
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