Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD
文献类型：期刊论文
| 作者 | Chen, Guang1,2; Li, Guoliang1,2; Song, Cuihua2; You, Jinmao1,2; Zhang, Xiaolong3 |
| 刊名 | JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 89期号:4页码:585-595 |
| 关键词 | Free Fatty Acids Hplc-fld Pre-column Derivatization Swertia Species Ultrasonic-assisted Extraction |
| ISSN号 | 0003-021X |
| 文献子类 | Article |
| 英文摘要 | Free fatty acids (FFA) are of the basic and indispensable components in medicinal plants. Many researches indicate the efficacy of medicinal plant require the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-Benzoacridine) ethyl-p-toluenesulfonate (BAETS) as labeling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C8 column (4.6 ×200 mm, 5 μm, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL-1, respectively. FFA analysis from 9 swertia species was performed by the newly developed method, and the FFA composition in these swertia samples was first reported.; Free fatty acids (FFA) are basic and indispensable components of medicinal plants. Many researches indicate that the efficacy of medicinal plant requires the presence of FFA. In the present study, ultrasonic-assisted extraction of FFA from Swertia species was optimized by response surface methodology (RSM), ensuring the highest FFA recoveries. A novel pre-column fluorescence labelling method using 2-(5-benzoacridine)ethyl-p-toluenesulfonate (BAETS) as a labelling reagent has been developed for highly sensitive and selective analysis of FFA by HPLC with fluorescent detection (FLD) and online mass spectrometry identification. RSM was also employed to optimize the fluorescence labelling of FFA. HPLC separation of 17 FFA derivatives was carried out on a reversed-phase Hypersil BDS C-8 column (4.6 x 200 mm, 5 mu m, Agilent Co.) with a gradient elution. The detection limits and quantifications were as low as 0.60 and 1.22 ng mL(-1), respectively. FFA analysis from nine Swertia species was performed by the newly developed method, and the FFA composition of these Swertia samples was first reported. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; IN-CAPILLARY DERIVATIZATION ; GAMMA-AMINOBUTYRIC-ACID ; ELECTROPHORETIC ANALYSIS ; CARBOXYLIC-ACIDS ; AMINO-ACIDS ; EXTRACTION ; REAGENT ; ALANINE ; OIL |
| WOS研究方向 | Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000305292300005 |
| 公开日期 | 2011-12-14 |
| 源URL | [http://ir.nwipb.ac.cn//handle/363003/1990]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Guang,Li, Guoliang,Song, Cuihua,et al. Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,2012,89(4):585-595. |
| APA | Chen, Guang,Li, Guoliang,Song, Cuihua,You, Jinmao,&Zhang, Xiaolong.(2012).Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD.JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,89(4),585-595. |
| MLA | Chen, Guang,et al."Composition Analysis of Free Fatty Acids from Swertia Species by a Novel Pre-column Fluorescence Labelling Method Using HPLC-FLD".JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 89.4(2012):585-595. |
入库方式： OAI收割
来源：西北高原生物研究所
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