Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS
文献类型：期刊论文
| 作者 | Ning, Shujing ; You, Jinmao ; Sun, Zhiwei ; Zhang, Shijuan ; Ji, Zhongyin |
| 刊名 | CHROMATOGRAPHIA
 ; Song, MH; Yu, FH; Ouyang, H; Cao, GM; Xu, XL; Cornelissen, JHC.Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS,CHROMATOGRAPHIA,2012,75(19-20):1107-1116
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| 出版日期 | 2012-10-01 |
| 英文摘要 | A simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (PASC) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. PASC reacted with aliphatic amines at 50 A degrees C for 4 min in aqueous acetonitrile (ACN) in the presence of sodiumtetraborate-NaOH buffer (0.10 mol L-1, pH 9.0) to give high yields of PASC-amine derivatives. Derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. The separation of derivatives was performed by a reversed-phase Hypersil BDS C8 column in combination with a gradient elution. The identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source in positive-ion detection mode. Excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. Comparing with 10-ethyl-acridine-2-sulfonyl chloride (EASC), PASC exhibited more intense fluorescence and ultraviolet absorbance. The proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples.; A simple and sensitive method for the determination of free aliphatic amines using 10-phenyl-acridone-2-sulfonyl chloride (PASC) as a labeling reagent by high-performance liquid chromatography with fluorescence detection and online mass spectrometry identification (HPLC-FLD-MS) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. PASC reacted with aliphatic amines at 50 A degrees C for 4 min in aqueous acetonitrile (ACN) in the presence of sodiumtetraborate-NaOH buffer (0.10 mol L-1, pH 9.0) to give high yields of PASC-amine derivatives. Derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 265 nm and an emission maximum at lambda(em) 418 nm. The separation of derivatives was performed by a reversed-phase Hypersil BDS C8 column in combination with a gradient elution. The identification of derivatives was carried out by online post-column mass spectrometry with atmospheric pressure chemical ionization (APCI) source in positive-ion detection mode. Excellent linear responses were observed with the correlation coefficients of larger than 0.9997, and detection limits (at a signal-to-noise of 3:1) were from 3.0 to 24.3 fmol. Comparing with 10-ethyl-acridine-2-sulfonyl chloride (EASC), PASC exhibited more intense fluorescence and ultraviolet absorbance. The proposed method is sensitive and reproducible for the determination of aliphatic amines from water and soil samples. |
| 源URL | [http://210.75.249.4/handle/363003/3606]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Ning, Shujing,You, Jinmao,Sun, Zhiwei,et al. Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS[J]. CHROMATOGRAPHIA, Song, MH; Yu, FH; Ouyang, H; Cao, GM; Xu, XL; Cornelissen, JHC.Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS,CHROMATOGRAPHIA,2012,75(19-20):1107-1116,2012. |
| APA | Ning, Shujing,You, Jinmao,Sun, Zhiwei,Zhang, Shijuan,&Ji, Zhongyin.(2012).Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS.CHROMATOGRAPHIA. |
| MLA | Ning, Shujing,et al."Fluorescence Probe of 10-Phenyl-acridone-2-sulfonyl Chloride and Its Application for Determination of Free Aliphatic Amines in Environmental Samples by HPLC with Fluorescence Detection and APCI-MS".CHROMATOGRAPHIA (2012). |
入库方式： OAI收割
来源：西北高原生物研究所
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