Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis
文献类型：期刊论文
| 作者 | Lv, Zhengxian ; You, Jinmao ; Lu, Shuaimin ; Sun, Weidi ; Ji, Zhongyin ; Sun, Zhiwei ; Song, Cuihua ; Chen, Guang ; Li, Guoliang ; Hu, Na ; Zhou, Wu ; Suo, Yourui |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017-03-31 |
| 英文摘要 | As the key aroma compounds, varietal thiols are the crucial odorants responsible for the flavor of wines. Quantitative analysis of thiols can provide crucial information for the aroma profiles of different wine styles. In this study, a rapid and sensitive method for the simultaneous determination of six thiols in wine using d(0)/d(4)-acridone-10-ethyl-N-maleimide (d(0)/d(4)-AENM) as stable isotope-coded derivatization reagent (SICD) by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) has been developed. Quantification of thiols was performed by using d(4)-AENM labeled thiols as the internal standards (IS), followed by stable isotope dilution HPLC-ESIMS/MS analysis. The AENM derivatization combined with multiple reactions monitoring (MRM) not only allowed trace analysis of thiols due to the extremely high sensitivity, but also efficiently corrected the matrix effects during HPLC-MS/MS and the fluctuation in MS/MS signal intensity due to instrument. The obtained internal standard calibration curves for six thiols were linear over the range of 25-10,000 pmol/L (R-2 >= 0.9961). Detection limits (LODs) for most of analytes were below 63 pmol/L. The proposed method was successfully applied for the simultaneous determination of six kinds of thiols in wine samples with precisions <3.5% and recoveries >78.1%. In conclusion, the developed method is expected to be a promising tool for detection of trace thiols in wine and also in other complex matrix. (C) 2017 Elsevier B.V. All rights reserved. |
| 源URL | [http://210.75.249.4/handle/363003/6827]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Lv, Zhengxian,You, Jinmao,Lu, Shuaimin,et al. Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2017. |
| APA | Lv, Zhengxian.,You, Jinmao.,Lu, Shuaimin.,Sun, Weidi.,Ji, Zhongyin.,...&Suo, Yourui.(2017).Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis.JOURNAL OF CHROMATOGRAPHY A. |
| MLA | Lv, Zhengxian,et al."Sensitive determination of thiols in wine samples by a stable isotope-coded derivatization reagent d(0)/d(4)-acridone-10-ethyl-N-maleimide coupled with high-performance liquid chrornatography-electrospray ionization-tandem mass spectrometry analysis".JOURNAL OF CHROMATOGRAPHY A (2017). |
入库方式： OAI收割
来源：西北高原生物研究所
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