中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography

文献类型:期刊论文

作者Zou, Denglang ; Chen, Tao ; Chen, Chen ; Li, Hongmei ; Liu, Yongling ; Li, Yulin
刊名JOURNAL OF CHROMATOGRAPHIC SCIENCE
出版日期2016-08-01
英文摘要In this article, macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for preparation of gallic acid from Terminalia bellirica (Gaertn.) Roxb. In the first step, six kinds of resins were investigated by adsorption and desorption tests and AB-8 macroporous resin was selected for the enrichment of gallic acid. As a result, 20 g of gallic acid at a purity of 71% could be separated from 100 g of crude extract in which the content of gallic acid was 16.7% and the recovery of gallic acid reached 85.0%. In the second step, preparative high-performance liquid chromatography was selected to purify gallic acid. As a result, 640 mg of gallic acid at a purity of 99.1% was obtained from 1 g of sample in 35 min. The results demonstrated that macroporous resin coupled with preparative high-performance liquid chromatography was suitable for preparation of gallic acid from T. bellirica (Gaertn.) Roxb.
源URL[http://210.75.249.4/handle/363003/6655]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Zou, Denglang,Chen, Tao,Chen, Chen,et al. An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016.
APA Zou, Denglang,Chen, Tao,Chen, Chen,Li, Hongmei,Liu, Yongling,&Li, Yulin.(2016).An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA Zou, Denglang,et al."An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2016).

入库方式: OAI收割

来源:西北高原生物研究所

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