An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
文献类型：期刊论文
| 作者 | Huang, Qiong ; Bu, Tong ; Zhang, Wentao ; Yan, Lingzhi ; Zhang, Mengyue ; Yang, Qingfeng ; Huang, Lunjie ; Yang, Baowei ; Hu, Na ; Suo, Yourui ; Wang, Jianlong ; Zhang, Daohong |
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2018-10-01 |
| 英文摘要 | Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors. |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://210.75.249.4/handle/363003/13818]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Qiong,Bu, Tong,Zhang, Wentao,et al. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier[J]. FOOD CHEMISTRY,2018. |
| APA | Huang, Qiong.,Bu, Tong.,Zhang, Wentao.,Yan, Lingzhi.,Zhang, Mengyue.,...&Zhang, Daohong.(2018).An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.FOOD CHEMISTRY. |
| MLA | Huang, Qiong,et al."An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier".FOOD CHEMISTRY (2018). |
入库方式： OAI收割
来源：西北高原生物研究所
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