Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)
文献类型:期刊论文
作者 | Chan, Antony C. S.1; Kim, Jinho1,4; Pan, An1,2; Xu, Han3,5; Nojima, Dana3,6; Hale, Christopher3; Wang, Songli3; Yang, Changhuei1 |
刊名 | SCIENTIFIC REPORTS
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出版日期 | 2019-07-31 |
卷号 | 9 |
ISSN号 | 2045-2322 |
DOI | 10.1038/s41598-019-47146-z |
产权排序 | 2 |
英文摘要 | We report the implementation of a parallel microscopy system (96 Eyes) that is capable of simultaneous imaging of all wells on a 96-well plate. The optical system consists of 96 microscopy units, where each unit is made out of a four element objective, made through a molded injection process, and a low cost CMOS camera chip. By illuminating the sample with angle varying light and applying Fourier Ptychography, we can improve the effective brig htfield imaging numerical aperture of the objectives from 0.23 to 0.3, and extend the depth of field from +/- 5 mu m to +/- 15 mu m. The use of Fourier Ptychography additionally allows us to computationally correct the objectives' aberrations out of the rendered images, and provides us with the ability to render phase images. The 96 Eyes acquires raw data at a rate of 0.7 frame per second (all wells) and the data are processed with 4 cores of graphical processing units (GPUs; GK210, Nvidia Tesla K80, USA). The system is also capable of fluorescence imaging (excitation = 465 nm, emission = 510 nm) at the native resolution of the objectives. We demonstrate the capability of this system by imaging S1P(1)-eGFP-Human bone osteosarcoma epithelial (U2OS) cells. |
语种 | 英语 |
WOS记录号 | WOS:000477950800037 |
出版者 | NATURE PUBLISHING GROUP |
源URL | [http://ir.opt.ac.cn/handle/181661/31625] ![]() |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Yang, Changhuei |
作者单位 | 1.CALTECH, Div Engn & Appl Sci, 1200 E Calif Blvd, Pasadena, CA 91125 USA 2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China 3.Amgen South San Francisco, 1120 Vet Blvd, San Francisco, CA 94080 USA 4.Samsung Engn, 26 Sangil Ro 6 Gil, Seoul, South Korea 5.A2 Biotherapeut, 2260 Townsgate Rd, Westlask Village, CA 91361 USA 6.Merck, 630 Gateway Blvd, San Francisco, CA 94080 USA |
推荐引用方式 GB/T 7714 | Chan, Antony C. S.,Kim, Jinho,Pan, An,et al. Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)[J]. SCIENTIFIC REPORTS,2019,9. |
APA | Chan, Antony C. S..,Kim, Jinho.,Pan, An.,Xu, Han.,Nojima, Dana.,...&Yang, Changhuei.(2019).Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes).SCIENTIFIC REPORTS,9. |
MLA | Chan, Antony C. S.,et al."Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)".SCIENTIFIC REPORTS 9(2019). |
入库方式: OAI收割
来源:西安光学精密机械研究所
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