中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)

文献类型:期刊论文

作者Chan, Antony C. S.1; Kim, Jinho1,4; Pan, An1,2; Xu, Han3,5; Nojima, Dana3,6; Hale, Christopher3; Wang, Songli3; Yang, Changhuei1
刊名SCIENTIFIC REPORTS
出版日期2019-07-31
卷号9
ISSN号2045-2322
DOI10.1038/s41598-019-47146-z
产权排序2
英文摘要

We report the implementation of a parallel microscopy system (96 Eyes) that is capable of simultaneous imaging of all wells on a 96-well plate. The optical system consists of 96 microscopy units, where each unit is made out of a four element objective, made through a molded injection process, and a low cost CMOS camera chip. By illuminating the sample with angle varying light and applying Fourier Ptychography, we can improve the effective brig htfield imaging numerical aperture of the objectives from 0.23 to 0.3, and extend the depth of field from +/- 5 mu m to +/- 15 mu m. The use of Fourier Ptychography additionally allows us to computationally correct the objectives' aberrations out of the rendered images, and provides us with the ability to render phase images. The 96 Eyes acquires raw data at a rate of 0.7 frame per second (all wells) and the data are processed with 4 cores of graphical processing units (GPUs; GK210, Nvidia Tesla K80, USA). The system is also capable of fluorescence imaging (excitation = 465 nm, emission = 510 nm) at the native resolution of the objectives. We demonstrate the capability of this system by imaging S1P(1)-eGFP-Human bone osteosarcoma epithelial (U2OS) cells.

语种英语
WOS记录号WOS:000477950800037
出版者NATURE PUBLISHING GROUP
源URL[http://ir.opt.ac.cn/handle/181661/31625]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Yang, Changhuei
作者单位1.CALTECH, Div Engn & Appl Sci, 1200 E Calif Blvd, Pasadena, CA 91125 USA
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian 710119, Shaanxi, Peoples R China
3.Amgen South San Francisco, 1120 Vet Blvd, San Francisco, CA 94080 USA
4.Samsung Engn, 26 Sangil Ro 6 Gil, Seoul, South Korea
5.A2 Biotherapeut, 2260 Townsgate Rd, Westlask Village, CA 91361 USA
6.Merck, 630 Gateway Blvd, San Francisco, CA 94080 USA
推荐引用方式
GB/T 7714
Chan, Antony C. S.,Kim, Jinho,Pan, An,et al. Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)[J]. SCIENTIFIC REPORTS,2019,9.
APA Chan, Antony C. S..,Kim, Jinho.,Pan, An.,Xu, Han.,Nojima, Dana.,...&Yang, Changhuei.(2019).Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes).SCIENTIFIC REPORTS,9.
MLA Chan, Antony C. S.,et al."Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes)".SCIENTIFIC REPORTS 9(2019).

入库方式: OAI收割

来源:西安光学精密机械研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。