Specific Imaging of Tyrosinase in Vivo with 3-Hydroxybenzyl Caged D-Luciferins
文献类型：期刊论文
| 作者 | Li, Shuang1,3; Hu, Rui1; Wang, Shuangqing1; Guo, Xudong1; Zeng, Yi2,3; Li, Yi2,3; Yang, Guoqiang1,3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018-08-07 |
| 卷号 | 90期号:15页码:9296-9300 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b01874 |
| 英文摘要 | Tyrosinase (TYR), a key enzyme in biosynthesis of melanin, usually functions as a biomarker of severe skin diseases such as vitiligo and melanoma cancer. Accurate detection of TYR activity in vivo is urgent but still challenging. Inspired by the advantages of bioluminescence in vivo strategy in imaging and the specific hydroxylation of 3-hydroxybenzyloxy group by TYR, a bioluminogenic probe, TYR-LH2, was designed and synthesized through caging D-luciferin with 3-hydroxybenzyl. The probe exhibits high selectivity and sensitivity toward TYR with a detection limit of 0.11 U/mL in a small detection volume of 100 mu L. Bioluminescence imaging results show that TYR-LH2 is fully competent for monitoring the dynamic changes of TYR in living cells and model animals and possesses the capability of discriminating melanocytes from other cell lines, thus offering a promising approach for investigation and diagnosis of melanoma cancer and other TYR-related diseases in vivo. |
| 语种 | 英语 |
| WOS记录号 | WOS:000441476600075 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/41909]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Hu, Rui; Yang, Guoqiang |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Photochem, Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Photochem Convers & Optoelect Mat, Tech Inst Phys & Chem, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuang,Hu, Rui,Wang, Shuangqing,et al. Specific Imaging of Tyrosinase in Vivo with 3-Hydroxybenzyl Caged D-Luciferins[J]. ANALYTICAL CHEMISTRY,2018,90(15):9296-9300. |
| APA | Li, Shuang.,Hu, Rui.,Wang, Shuangqing.,Guo, Xudong.,Zeng, Yi.,...&Yang, Guoqiang.(2018).Specific Imaging of Tyrosinase in Vivo with 3-Hydroxybenzyl Caged D-Luciferins.ANALYTICAL CHEMISTRY,90(15),9296-9300. |
| MLA | Li, Shuang,et al."Specific Imaging of Tyrosinase in Vivo with 3-Hydroxybenzyl Caged D-Luciferins".ANALYTICAL CHEMISTRY 90.15(2018):9296-9300. |
入库方式： OAI收割
来源：化学研究所
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