Hydroxyethyl-functionalized ultrasmall chitosan nanoparticles as a gene delivery carrier
文献类型：期刊论文
| 作者 | Du, Libo1; Gao, Yanli1; Yang, Hui2,3; Li, Yanchao1; Zhuang, Qianfen1; Jia, Hongying1; Nie, Guangjun2; Liu, Yang1 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2013 |
| 卷号 | 3期号:34页码:14791-14797 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c3ra42100g |
| 英文摘要 | A non-viral gene vector based on hydroxyethyl ultra-low molecular weight chitosan nanoparticles (HE-ULMWCh NPs) has been synthesized. The HE-ULMWCh is used to form nanoparticles with an enhanced green fluorescence protein (pEGFP) encoding plasmid that possesses diameters of 30-50 nm and a molecular weight of less than 2 kDa. The cytotoxicity assay shows that this new gene vector is significantly less cytotoxic than polyethylenimine whilst still retaining the characteristics of a cationic polyelectrolyte. Cellular uptake of HE-ULMWCh NP-pDNA nanocomplexes shows that HE-ULMWCh NPs can readily enter into cells via endocytosis and then be delivered to lysosomes. pDNA can be easily released from HE-ULMWCh NPs in the acidic vesicles of lysosomes, therefore resulting in a significant increase in the transfection efficiency in three different cell lines compared to naked pDNA. The in vitro results using the new gene vector are further confirmed by in vivo transfection in which HE-ULMWCh NPs provided 3.2 fold greater transfection efficiency than naked pDNA. The HE-ULMWCh NPs can therefore be concluded to be a promising delivery system for in vitro and in vivo gene transfection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000322737400076 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42006]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Yang |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Ctr Mol Sci, Beijing 100190, Peoples R China 2.Natl Ctr Nanosci & Technol China, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.China Acad Chinese Med Sci, Guanganmen Hosp, Dept Immunol, Beijing 100053, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Libo,Gao, Yanli,Yang, Hui,et al. Hydroxyethyl-functionalized ultrasmall chitosan nanoparticles as a gene delivery carrier[J]. RSC ADVANCES,2013,3(34):14791-14797. |
| APA | Du, Libo.,Gao, Yanli.,Yang, Hui.,Li, Yanchao.,Zhuang, Qianfen.,...&Liu, Yang.(2013).Hydroxyethyl-functionalized ultrasmall chitosan nanoparticles as a gene delivery carrier.RSC ADVANCES,3(34),14791-14797. |
| MLA | Du, Libo,et al."Hydroxyethyl-functionalized ultrasmall chitosan nanoparticles as a gene delivery carrier".RSC ADVANCES 3.34(2013):14791-14797. |
入库方式： OAI收割
来源：化学研究所
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