Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers
文献类型：期刊论文
| 作者 | Xing BaoLing1; Song JinZhao2; Ge SuMei1; Tang ZhengHua1; Liu MengLu1; Yang Qiong2; Lu FengTing2; Liu LiBing2; Wang Shu2 |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2013-03-01 |
| 卷号 | 58期号:8页码:873-878 |
| 关键词 | Conjugated Polymers Dna Mutation Kras Braf Sensors |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-012-5553-9 |
| 英文摘要 | The mutation detections of KRAS and BRAF genes are of significant importance to predict the responses to anti-cancer therapy and develop new drugs. In this paper, we developed a multi-step fluorescence resonance energy transfer (FRET) assay for multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers (CCP). The newly established detection system could detect as low as 2% mutant DNAs in DNA admixtures. By triggering the emission intensity change of CCP and the dyes labeled in the DNA, four possible statuses (three mutations and one wildtype) can be differentiated in one extension reaction. The detection efficiency of this new method in clinical molecular diagnosis was validated by determining KRAS and BRAF mutations of 51 formalin-fixed paraffin-embedded (FFPE) ovary tissue samples. Furthermore, the result of the CCP-based multi-step FRET assay can be directly visualized under UV light so that no expensive instruments and technical expertise are needed. Thus, the assay provides a sensitive, reliable, cost-effective and simple method for the detection of disease-related gene mutations. |
| 语种 | 英语 |
| WOS记录号 | WOS:000316014600005 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42088]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang Qiong |
| 作者单位 | 1.Changzhou Wommen & Children Hlth Hosp, Dept Pathol, Changzhou 213003, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xing BaoLing,Song JinZhao,Ge SuMei,et al. Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers[J]. CHINESE SCIENCE BULLETIN,2013,58(8):873-878. |
| APA | Xing BaoLing.,Song JinZhao.,Ge SuMei.,Tang ZhengHua.,Liu MengLu.,...&Wang Shu.(2013).Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers.CHINESE SCIENCE BULLETIN,58(8),873-878. |
| MLA | Xing BaoLing,et al."Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers".CHINESE SCIENCE BULLETIN 58.8(2013):873-878. |
入库方式： OAI收割
来源：化学研究所
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