Microchip CE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor based on gold nanoparticle
文献类型：期刊论文
| 作者 | Qiao, Juan1; Qi, Li1; Yan, Huijuan2; Li, Yaping1,3; Mu, Xiaoyu1,3 |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2013-02-01 |
| 卷号 | 34期号:3页码:409-416 |
| 关键词 | L-asparaginase Enzyme Reactor L-glutamine Gold Nanoparticle |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201200461 |
| 英文摘要 | l-Asparaginase (l-Asnase) can suppress the growth of malignant cells by rapid depletion of two essential amino acids, l-glutamine (l-Gln) and l-asparagine (l-Asn). To study the cytotoxic effect and the secondary complications of l-Asnase in the treatment of acute lymphoblastic leukemia, the development of a novel enzyme reactor of l-Asnase for the hydrolysis of l-Gln, employing the enzyme-gold nanoparticle conjugates in capillary, was reported in this work. First, a microchip CE (MCE)-LIF was established for the separation of l-amino acids (l-Gln and l-glutamic acid) and studying the hydrolysis of l-Gln by using l-Asnase enzyme reactor. Then, using l-Gln as target analyte, the enzyme kinetics of l-Asnase in free solution, enzyme-gold nanoparticle conjugates (E-GNP), and the enzyme-gold nanoparticle conjugates immobilized in capillary (E-GNP-C) were investigated in detail with the proposed MCE-LIF method. Moreover, for optimizing the enzymatic reaction efficiency, three important parameters, including the length of capillary, the enzyme concentration reacted with gold nanoparticle and the amount of l-Asnase immobilized on the gold nanoparticle, have been studied. Owing to the high specific activity, the E-GNP-C enzyme reactor exhibited the best performance for the hydrolysis of l-Gln. |
| 语种 | 英语 |
| WOS记录号 | WOS:000313807300010 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42114]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Juan,Qi, Li,Yan, Huijuan,et al. Microchip CE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor based on gold nanoparticle[J]. ELECTROPHORESIS,2013,34(3):409-416. |
| APA | Qiao, Juan,Qi, Li,Yan, Huijuan,Li, Yaping,&Mu, Xiaoyu.(2013).Microchip CE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor based on gold nanoparticle.ELECTROPHORESIS,34(3),409-416. |
| MLA | Qiao, Juan,et al."Microchip CE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor based on gold nanoparticle".ELECTROPHORESIS 34.3(2013):409-416. |
入库方式： OAI收割
来源：化学研究所
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