3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells
文献类型：期刊论文
| 作者 | Lu, Jinxin; Song, Yanchao; Shi, Wen; Li, Xiaohua; Ma, Huimin |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2013-04-01 |
| 卷号 | 31期号:4页码:472-478 |
| 关键词 | Glutathione S-transferase Fluorescence Nanoprobe Quantum Dots Bioimaging |
| ISSN号 | 1001-604X |
| DOI | 10.1002/cjoc.201300061 |
| 英文摘要 | A novel fluorescent nanoprobe for glutathione S-transferase (GST) has been developed by incorporating 3,4-dinitrobenzamide (a specific substrate of GST) onto CdTe/ZnTe quantum dots. The probe itself displays a low background signal due to the strong quenching effect of the electron-withdrawing unit of 3,4-dinitrobenzamide on the quantum dots. However, GST can efficiently catalyze the nucleophilic substitution of reduced glutathione on the p-nitro group of the nanoprobe, leading to a large fluorescence enhancement. Most notably, this enhancement shows high selectivity and sensitivity towards GST instead of the other biological substances. With this nanoprobe, a simple fluorescence imaging method for intracellular GST has been established, and its applicability has been successfully demonstrated for imaging GST in different living cells, which reveals that A549 cells express GST about 3 times higher than NIH-3T3 and Hela cells. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317892800008 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42135]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shi, Wen |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Jinxin,Song, Yanchao,Shi, Wen,et al. 3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells[J]. CHINESE JOURNAL OF CHEMISTRY,2013,31(4):472-478. |
| APA | Lu, Jinxin,Song, Yanchao,Shi, Wen,Li, Xiaohua,&Ma, Huimin.(2013).3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells.CHINESE JOURNAL OF CHEMISTRY,31(4),472-478. |
| MLA | Lu, Jinxin,et al."3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells".CHINESE JOURNAL OF CHEMISTRY 31.4(2013):472-478. |
入库方式： OAI收割
来源：化学研究所
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