A label-free electrochemical biosensor based on a DNA aptamer against codeine
文献类型：期刊论文
| 作者 | Huang, Liangliang1; Yang, Xiaojuan2; Qi, Cui2; Niu, Xiaofang1; Zhao, Chunling1; Zhao, Xiaohui1; Shangguan, Dihua2; Yang, Yunhui1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2013-07-17 |
| 卷号 | 787页码:203-210 |
| 关键词 | Codeine Aptamer Systematic Evolution Of Ligands By Exponential Enrichment Electrochemical Aptasensor Au-mesoporous Silica Nanoparticles |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2013.05.024 |
| 英文摘要 | In order to develop a sensor for opium alkaloid codeine detection, DNA aptamers against codeine were generated by SELEX (systematic evolution of ligands by exponential enrichment) technique. An aptamer HL7-14, which is a 37-mer sequence with K-d values of 0.91 mu M, was optimized by the truncation-mutation assay. The specificity investigation shows that HL7-14 exhibits high specificity to codeine over morphine, and almost cannot bind to other small molecule. With this new selected aptamer, a novel electrochemical label-free codeine aptamer biosensor based on Au-mesoporous silica nanoparticles (Au-MSN) as immobilized substrate has been proposed using [Fe(CN)(6)](3-/4-) as electroactive redox probe. The linear range covered from 10 pM to 100 nM with correlation coefficient of 0.9979 and the detection limit was 3 pM. Our study demonstrates that the biosensor has good specificity, stability and well regeneration. It can be used to detect codeine. (C) 2013 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000321497400025 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42220]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Shangguan, Dihua |
| 作者单位 | 1.Yunnan Normal Univ, Chem & Chem Engn Coll, Kunming 650092, Peoples R China 2.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Liangliang,Yang, Xiaojuan,Qi, Cui,et al. A label-free electrochemical biosensor based on a DNA aptamer against codeine[J]. ANALYTICA CHIMICA ACTA,2013,787:203-210. |
| APA | Huang, Liangliang.,Yang, Xiaojuan.,Qi, Cui.,Niu, Xiaofang.,Zhao, Chunling.,...&Yang, Yunhui.(2013).A label-free electrochemical biosensor based on a DNA aptamer against codeine.ANALYTICA CHIMICA ACTA,787,203-210. |
| MLA | Huang, Liangliang,et al."A label-free electrochemical biosensor based on a DNA aptamer against codeine".ANALYTICA CHIMICA ACTA 787(2013):203-210. |
入库方式： OAI收割
来源：化学研究所
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