Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification
文献类型：期刊论文
| 作者 | Duan, Ruixue1; Zuo, Xiaolei2; Wang, Shutao3; Quan, Xiyun4; Chen, Dongliang4; Chen, Zhifei1; Jiang, Lei3; Fan, Chunhai2; Xia, Fan1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2013-03-27 |
| 卷号 | 135期号:12页码:4604-4607 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/ja311313b |
| 英文摘要 | Through rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 degrees C and 1 aM at 4 degrees C, which corresponds to nine strands of miR-21 in a 15 mu L sample, and it is capable of distinguishing among miRNA family members. More importantly, the proposed approach is also sensitive and selective when applied to crude extractions from MCF-7 and PC3 cell lines and even patient tissues from intraductal carcinoma and invasive ductal carcinoma of the breast. |
| 语种 | 英语 |
| WOS记录号 | WOS:000317032000008 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42256]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xia, Fan |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China 4.Zhuzhou 1 Hosp, Dept Pathol, Zhuzhou 412000, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Ruixue,Zuo, Xiaolei,Wang, Shutao,et al. Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(12):4604-4607. |
| APA | Duan, Ruixue.,Zuo, Xiaolei.,Wang, Shutao.,Quan, Xiyun.,Chen, Dongliang.,...&Xia, Fan.(2013).Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(12),4604-4607. |
| MLA | Duan, Ruixue,et al."Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.12(2013):4604-4607. |
入库方式： OAI收割
来源：化学研究所
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