Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing
文献类型：期刊论文
| 作者 | Liu, Lei1,2; Li, Ting2; Zhang, Shouwen3; Song, Peng4; Guo, Bingyuan1,2; Zhao, Yuliang2; Wu, Hai-Chen1 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2018-09-10 |
| 卷号 | 57期号:37页码:11882-11887 |
| 关键词 | Antigens Barcode Dna Biomarkers Multiplexed Detection Nanopore Sensing |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201803324 |
| 英文摘要 | Protein biomarkers in blood have been widely used in the early diagnosis of disease. However, simultaneous detection of many biomarkers in a single sample remains challenging. Herein, we show that the combination of a sandwich assay and DNA-assisted nanopore sensing could unambiguously identify and quantify several antigens in a mixture. We use five barcode DNAs to label different gold nanoparticles that can selectively bind specific antigens. After the completion of the sandwich assay, barcode DNAs are released and subject to nanopore translocation tests. The distinct current signatures generated by each barcode DNA allow simultaneous quantification of biomarkers at picomolar level in clinical samples. This approach would be very useful for accurate and multiplexed quantification of cancer-associated biomarkers within a very small sample volume, which is critical for non-invasive early diagnosis of cancer. |
| 语种 | 英语 |
| WOS记录号 | WOS:000443675700003 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42309]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Lei; Wu, Hai-Chen |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China 3.Beijing ChaoYang Emergency Med Ctr, Epilepsy Dept, Beijing 100021, Peoples R China 4.Gen Hosp Chinese Peoples Liberat Army, Dept Geriatr Oncol, Beijing 100853, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lei,Li, Ting,Zhang, Shouwen,et al. Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(37):11882-11887. |
| APA | Liu, Lei.,Li, Ting.,Zhang, Shouwen.,Song, Peng.,Guo, Bingyuan.,...&Wu, Hai-Chen.(2018).Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(37),11882-11887. |
| MLA | Liu, Lei,et al."Simultaneous Quantification of Multiple Cancer Biomarkers in Blood Samples through DNA-Assisted Nanopore Sensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.37(2018):11882-11887. |
入库方式： OAI收割
来源：化学研究所
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