Urea and plasma ice-nucleating proteins promoted the modest freeze tolerance in Pleske's high altitude frog Nanorana pleskei
文献类型：期刊论文
| 作者 | Niu, Yonggang1; Wang, Jianjun2; Men, Shengkang1; Zhao, Yaofeng1; Lu, Songsong1; Tang, Xiaolong1; Chen, Qiang1 |
| 刊名 | JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 188期号:4页码:599-610 |
| ISSN号 | 0174-1578 |
| 关键词 | Cryoprotectants Urea Ice-nucleating Protein Freeze Tolerance Nanorana Pleskei |
| DOI | 10.1007/s00360-018-1159-0 |
| 英文摘要 | The frog Nanorana pleskei (Dicroglossidae) is indigenous to the Qinghai-Tibetan Plateau. To identify its strategies in coping with the cold climate, we measured the hibernacula microhabitat temperature during winter. We also examined the freezing-induced and seasonal variation of several putative cryoprotectants in the heart, liver, brain, kidney and muscle, as well as ice-nucleating protein in plasma. Our results showed that N. pleskei survived exposure to temperatures as low as - 2.5 +/- 0.40 A degrees C during hibernation, which was lower than the body fluid freezing point (- 0.43 +/- 0.01 A degrees C). Experimental freezing results indicated that four of six specimens could survive 12 h of freezing at - 2 A degrees C with 27.5 +/- 2.5% of total body water as ice. Concomitantly, the water contents of all examined organs decreased after being frozen for 24 h at - 2 A degrees C. The levels of urea in heart significantly increased from 71.05 +/- 7.19 to 104.59 +/- 10.11 A mu mol g(-1), and in muscle increased from 72.23 +/- 3.40 to 102.42 +/- 6.24 A mu mol g(-1) when exposed to freezing; other cryoprotectants (glucose, glycerol, and lactate) showed no significant increase in all examined tissues. In addition, urea levels were significantly higher in fall-collected frogs than summer-collected frogs in the tissues of heart, brain, kidney, and muscle. The results of differential scanning calorimetry indicated that the ice-nucleating protein was present only in cold-acclimated and fall-collected frogs' plasma. We concluded that the urea serves as a primary cryoprotectant and accumulates in anticipation of freezing in N. pleskei, coupling with the seasonal production of plasma ice-nucleating protein. |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:000435594800004 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42543]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Qiang |
| 作者单位 | 1.Lanzhou Univ, Inst Biochem & Mol Biol, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Yonggang,Wang, Jianjun,Men, Shengkang,et al. Urea and plasma ice-nucleating proteins promoted the modest freeze tolerance in Pleske's high altitude frog Nanorana pleskei[J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2018,188(4):599-610. |
| APA | Niu, Yonggang.,Wang, Jianjun.,Men, Shengkang.,Zhao, Yaofeng.,Lu, Songsong.,...&Chen, Qiang.(2018).Urea and plasma ice-nucleating proteins promoted the modest freeze tolerance in Pleske's high altitude frog Nanorana pleskei.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,188(4),599-610. |
| MLA | Niu, Yonggang,et al."Urea and plasma ice-nucleating proteins promoted the modest freeze tolerance in Pleske's high altitude frog Nanorana pleskei".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 188.4(2018):599-610. |
入库方式： OAI收割
来源：化学研究所
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