Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA
文献类型：期刊论文
| 作者 | Qin, Tingxiao1,2,3; Liu, Kunhui2; Song, Di1; Yang, Chunfan2; Zhao, Hongmei1; Su, Hongmei2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 19期号:4 |
| 关键词 | Z-dna Zntmpyp4 Transient Absorption Spectroscopy Binding Mode |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms19041071 |
| 英文摘要 | Recognition of unusual left-handed Z-DNA by specific binding of small molecules is crucial for understanding biological functions in which this particular structure participates. Recent investigations indicate that zinc cationic porphyrin (ZnTMPyP4) is promising as a probe for recognizing Z-DNA due to its characteristic chiroptical properties upon binding with Z-DNA. However, binding mechanisms of the ZnTMPyP4/Z-DNA complex remain unclear. By employing time-resolved UV-visible absorption spectroscopy in conjunction with induced circular dichroism (ICD), UV-vis, and fluorescence measurements, we examined the binding interactions of ZnTMPyP4 towards B-DNA and Z-DNA. For the ZnTMPyP4/Z-DNA complex, two coexisting binding modes were identified as the electrostatic interaction between pyridyl groups and phosphate backbones, and the major groove binding by zinc(II) coordinating with the exposed guanine N7. The respective contribution of each mode is assessed, allowing a complete scenario of binding modes revealed for the ZnTMPyP4/Z-DNA. These interaction modes are quite different from those (intercalation and partial intercalation modes) for the ZnTMPyP4/B-DNA complex, thereby resulting in explicit differentiation between B-DNA and Z-DNA. Additionally, the binding interactions of planar TMPyP4 to DNA were also investigated as a comparison. It is shown that without available virtual orbitals to coordinate, TMPyP4 binds with Z-DNA solely in the intercalation mode, as with B-DNA, and the intercalation results in a structural transition from Z-DNA to B-ZNA. These results provide mechanistic insights for understanding ZnTMPyP4 as a probe of recognizing Z-DNA and afford a possible strategy for designing new porphyrin derivatives with available virtual orbitals for the discrimination of B-DNA and Z-DNA. |
| 语种 | 英语 |
| WOS记录号 | WOS:000434978700150 |
| 出版者 | MDPI |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42563]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Song, Di; Su, Hongmei |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China 2.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Tingxiao,Liu, Kunhui,Song, Di,et al. Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(4). |
| APA | Qin, Tingxiao,Liu, Kunhui,Song, Di,Yang, Chunfan,Zhao, Hongmei,&Su, Hongmei.(2018).Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(4). |
| MLA | Qin, Tingxiao,et al."Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.4(2018). |
入库方式： OAI收割
来源：化学研究所
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