A time-dependent fluorescent biosensor for uracil-DNA glycosylase detection based on the uracil inhibition effect towards archaebacterial DNA polymerases
文献类型：期刊论文
| 作者 | Zhang, Xuzhe1; Wu, Tongbo3,5; Wang, Hongbo4; Zou, Yufan1; Chen, Wei3; Zhao, Meiping3; Wang, Shanshan2; Xiao, Xianjin1 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2018-10-01 |
| 卷号 | 270页码:277-282 |
| ISSN号 | 0925-4005 |
| 关键词 | Uracil-dna Glycosylase Archaebacterial Dna Polymerase Uracil Inhibition Time-dependent Fluorescent Detection Exponential Amplification |
| DOI | 10.1016/j.snb.2018.05.041 |
| 英文摘要 | Uracil-DNA glycosylase (UDG) plays important role in DNA lesion repair. It can remove the uracil lesion in DNA and is involved in multiple biological processes. UDG activity detection methods based on DNA probe are developed fast recently due to its high sensitivity and simplicity. They typically use two strategies: the conformational change or the local environmental change of the DNA probe after UDG cleavage. Here we proposed a new strategy for UDG activity detection, which utilized uracil-containing oligo to inhibit archaebacterial DNA polymerases, and after UDG treatment the archaebacterial DNA polymerases could be activated to generate linear or exponential amplification fluorescent signal. The exponential methods showed a time-dependent signal output and achieved limit of detection of 0.0025 U/mL. More importantly, the feature of time-dependence allowed us to easily modulate the resolution or the dynamic range of our method to meet different demands in various occasions. The strategy was also demonstrated to be highly selective toward DNase and exonucleases and had been applied to detect the UDG activity in cell lysate. |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000434011500034 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42645]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shanshan; Xiao, Xianjin |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Family Planning Res Inst, Tongji Med Coll, Ctr Reprod Med, Wuhan 430030, Hubei, Peoples R China 2.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Safety & Qual, Beijing 100081, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 4.Huazhong Univ Sci & Technol, Union Hosp, Dept Obstet & Gynaecol, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China 5.Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2G3, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Xuzhe,Wu, Tongbo,Wang, Hongbo,et al. A time-dependent fluorescent biosensor for uracil-DNA glycosylase detection based on the uracil inhibition effect towards archaebacterial DNA polymerases[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,270:277-282. |
| APA | Zhang, Xuzhe.,Wu, Tongbo.,Wang, Hongbo.,Zou, Yufan.,Chen, Wei.,...&Xiao, Xianjin.(2018).A time-dependent fluorescent biosensor for uracil-DNA glycosylase detection based on the uracil inhibition effect towards archaebacterial DNA polymerases.SENSORS AND ACTUATORS B-CHEMICAL,270,277-282. |
| MLA | Zhang, Xuzhe,et al."A time-dependent fluorescent biosensor for uracil-DNA glycosylase detection based on the uracil inhibition effect towards archaebacterial DNA polymerases".SENSORS AND ACTUATORS B-CHEMICAL 270(2018):277-282. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。