Molecular mechanics of coiled coils loaded in the shear geometry
文献类型：期刊论文
| 作者 | Goktas, Melis1; Luo, Chuanfu2,3; Sullan, Ruby May A.1,4; Bergues-Pupo, Ana E.2; Lipowsky, Reinhard2; Verde, Ana Vila2; Blank, Kerstin G.1 |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2018-05-28 |
| 卷号 | 9期号:20页码:4610-4621 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c8sc01037d |
| 英文摘要 | Coiled coils are important nanomechanical building blocks in biological and biomimetic materials. A mechanistic molecular understanding of their structural response to mechanical load is essential for elucidating their role in tissues and for utilizing and tuning these building blocks in materials applications. Using a combination of single-molecule force spectroscopy (SMFS) and steered molecular dynamics (SMD) simulations, we have investigated the mechanics of synthetic heterodimeric coiled coils of different length (3-4 heptads) when loaded in shear geometry. Upon shearing, we observe an initial rise in the force, which is followed by a constant force plateau and ultimately strand separation. The force required for strand separation depends on the coiled coil length and the applied loading rate, suggesting that coiled coil shearing occurs out of equilibrium. This out-of-equilibrium behaviour is determined by a complex structural response which involves helix uncoiling, uncoiling-assisted sliding of the helices relative to each other in the direction of the applied force as well as uncoiling-assisted dissociation perpendicular to the force axis. These processes follow a hierarchy of timescales with helix uncoiling being faster than sliding and sliding being faster than dissociation. In SMFS experiments, strand separation is dominated by uncoiling-assisted dissociation and occurs at forces between 25-45 pN for the shortest 3-heptad coiled coil and between 35-50 pN for the longest 4-heptad coiled coil. These values are highly similar to the forces required for shearing apart short double-stranded DNA oligonucleotides, reinforcing the potential role of coiled coils as nanomechanical building blocks in applications where protein-based structures are desired. |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000433426700008 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42707]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Verde, Ana Vila; Blank, Kerstin G. |
| 作者单位 | 1.Max Planck Inst Colloids & Interfaces, Mechanobio Chem, Sci Pk Potsdam Golm, D-14424 Potsdam, Germany 2.Max Planck Inst Colloids & Interfaces, Dept Theory & Biosyst, Sci Pk Potsdam Golm, D-14424 Potsdam, Germany 3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China 4.Univ Toronto Scarborough, Dept Phys & Environm Sci, 1265 Mil Trail, Toronto, ON M1C 1A4, Canada |
| 推荐引用方式 GB/T 7714 | Goktas, Melis,Luo, Chuanfu,Sullan, Ruby May A.,et al. Molecular mechanics of coiled coils loaded in the shear geometry[J]. CHEMICAL SCIENCE,2018,9(20):4610-4621. |
| APA | Goktas, Melis.,Luo, Chuanfu.,Sullan, Ruby May A..,Bergues-Pupo, Ana E..,Lipowsky, Reinhard.,...&Blank, Kerstin G..(2018).Molecular mechanics of coiled coils loaded in the shear geometry.CHEMICAL SCIENCE,9(20),4610-4621. |
| MLA | Goktas, Melis,et al."Molecular mechanics of coiled coils loaded in the shear geometry".CHEMICAL SCIENCE 9.20(2018):4610-4621. |
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来源：化学研究所
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