A Rapid Colorimetric Method to Visualize Protein Interactions
文献类型：期刊论文
| 作者 | Liu, Bing1,3; Wang, Zhihao3; Lan, Ling4; Yang, Qianfan5; Zhang, Peipei1; Shi, Lei2; Lang, Yunhe2; Tabib-Salazar, Aline3; Wigneshweraraj, Sivaramesh3; Zhang, Jiye1 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2018-05-07 |
| 卷号 | 24期号:26页码:6727-6731 |
| 关键词 | Colorirnetry Nmr Spectroscopy Protein-protein Interactions Supramolecular Chemistry |
| ISSN号 | 0947-6539 |
| DOI | 10.1002/chem.201800401 |
| 英文摘要 | As key molecules in most biological pathways, proteins physically contact one or more biomolecules in a highly specific manner. Several driving forces (i.e., electrostatic and hydrophobic) facilitate such interactions and a variety of methods have been developed to monitor these processes both in vivo and in vitro. In this work, a new method is reported for the detection of protein interactions by visualizing a color change of a cyanine compound, a supramolecule complex of 3,3-di-(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC). Nuclear magnetic resonance (NMR) studies suggest that the hydrophobic nature of the protein surfaces drives MTC into different types of aggregates with distinct colors. When proteins interact with other biomolecules, the hydrophobic surface of the complex differs, resulting in a shift in the form of MTC aggregation, which results in a color change. As a result, this in vitro method has the potential to become a rapid tool for the confirmation of protein-biomolecule interactions, without the requirements for sophisticated instrumentation or approaches. |
| 语种 | 英语 |
| WOS记录号 | WOS:000431492500009 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42767]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Matthews, Steve; Zhang, Xiufeng |
| 作者单位 | 1.Xi An Jiao Tong Univ, BioBank, Affiliated Hosp 1, Sch Med, Xian 710049, Shaanxi, Peoples R China 2.North China Univ Sci & Technol, Coll Chem Engn, Tangshan 063210, Peoples R China 3.Imperial Coll London, Prof Dr S Matthews Dept Life Sci, London, England 4.Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Inst Chem, Beijing 100190, Peoples R China 5.Sichuan Univ, Coll Chem, Chengdu 610065, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Bing,Wang, Zhihao,Lan, Ling,et al. A Rapid Colorimetric Method to Visualize Protein Interactions[J]. CHEMISTRY-A EUROPEAN JOURNAL,2018,24(26):6727-6731. |
| APA | Liu, Bing.,Wang, Zhihao.,Lan, Ling.,Yang, Qianfan.,Zhang, Peipei.,...&Zhang, Xiufeng.(2018).A Rapid Colorimetric Method to Visualize Protein Interactions.CHEMISTRY-A EUROPEAN JOURNAL,24(26),6727-6731. |
| MLA | Liu, Bing,et al."A Rapid Colorimetric Method to Visualize Protein Interactions".CHEMISTRY-A EUROPEAN JOURNAL 24.26(2018):6727-6731. |
入库方式： OAI收割
来源：化学研究所
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