Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior
文献类型：期刊论文
| 作者 | Li, Zhao1,2,3; Huang, Sha1,2,3; Liu, Yufan1,2,3; Yao, Bin2,3,4; Hu, Tian2,3,4; Shi, Haigang5; Xie, Jiangfan2,3; Fu, Xiaobing1,2,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018-05-22 |
| 卷号 | 8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-26407-3 |
| 英文摘要 | Bioink optimization is considered as one of main challenges in cell-laden 3D bioprinting. Alginate-Gelatin (Alg-Gel) hydrogel have been extensively used as bioink. However, its properties could be influenced by various parameters, and little is known about the evidence featuring the impact of solvent. Here we investigated four Alg-Gel bioink by varying solvent ionic strength (named B-1, B-2, B-3 and B-4). Mechanical properties and printability of bioink samples and their impacts on behaviors of encapsulated epidermal stem cells (ESCs) were tested. Bioink with increased ionic strength of solvent showed decreased stiffness and viscosity, and increased swelling and degradation by printability and mechanical property tests. Due to the increased swelling and degradation was associated with shape-maintenance of post-printing constructs, B-3 and B-4 were hardly observable after 14 days. Cellular behaviors were assessed through viability, proliferation, aggregation and differentiation tests. B-2 with optimal properties resulted in higher viability and proliferation of ESCs, and further facilitated cellular aggregation and lineage differentiation. We demonstrated that the solvent can be tuned by ionic strength to control the properties of Alg-Gel bioink and post-printing constructs, which represented a promising avenue for promotion of therapeutic stem cell behaviors in 3D bioprinting. |
| 语种 | 英语 |
| WOS记录号 | WOS:000432656900001 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/42843]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Huang, Sha; Fu, Xiaobing |
| 作者单位 | 1.Gen Hosp PLA, Inst Basic Med Sci, Beijing, Peoples R China 2.Gen Hosp PLA, Key Lab Tissue Repair & Regenerat PLA, Hosp 1, Beijing, Peoples R China 3.Gen Hosp PLA, Hosp 1, Beijing Key Res Lab Skin Injury Repair & Regenera, Beijing, Peoples R China 4.Nankai Univ, Med Coll, Tianjin, Peoples R China 5.Chinese Acad Sci, Tech Inst Phys & Chem, Natl Res Ctr Engn Plast, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Zhao,Huang, Sha,Liu, Yufan,et al. Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Li, Zhao.,Huang, Sha.,Liu, Yufan.,Yao, Bin.,Hu, Tian.,...&Fu, Xiaobing.(2018).Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior.SCIENTIFIC REPORTS,8. |
| MLA | Li, Zhao,et al."Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior".SCIENTIFIC REPORTS 8(2018). |
入库方式： OAI收割
来源：化学研究所
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