Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study
文献类型：期刊论文
| 作者 | Zhao, Liping1,4; Qiao, Juan1,2; Zhang, Ke2,3; Li, Dan5; Zhang, Hongyi4; Qi, Li1,2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2018-05-04 |
| 卷号 | 1548页码:104-110 |
| 关键词 | Chiral Ligand Exchange Capillary Electrochromatography Enantioseparation Block Copolymer Coating Enzyme Kinetics Study |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2018.03.031 |
| 英文摘要 | A chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and implemented for D,L-amino acids enantioseparation with poly(maleic anhydride-styrene-methacryloylL-arginine methyl ester) as the coating. The block copolymer was synthesized through the reversible addition fragmentation chain transfer reaction. In the constructed CLE-CEC system, poly (methacryloylL-arginine methyl ester) moiety of the block copolymer played the role as the immobilized chiral ligand and Zn (II) was used as the central ion. Key factors, including pH of buffer solution, ratio of Zn (II) to ligands, the mass ratio of monomers in the block copolymer, which affect the enantioresolution were investigated. Comparing with the bare capillary, the CLE-CEC enantioresolution was enhanced greatly with the coating one. 5 Pairs of D,L-amino acids enantiomers obtained baseline separation with 5 pairs partly separated. The mechanism of enhancement enantioresolution of the developed CLE-CEC system was explored briefly. Further, good linearities were achieved in the range of 25.0 mu M-5.0 mM for quantitative analysis of D-glutamine (r(2) = 0.997) and L-glutamine (r(2) = 0.991). Moreover, the proposed CLE-CEC assay was successfully applied in the kinetics study of glutaminase by using L-glutamine as the substrate. (C) 2018 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000430779500013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43131]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Hongyi; Qi, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China 4.Hebei Univ, Key Lab Analyt Sci & Technol Hebei Prov, Coll Chem & Environm Sci, 180 Wusi East Rd, Baoding 071002, Peoples R China 5.Jinan Univ, Guangzhou 510632, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Liping,Qiao, Juan,Zhang, Ke,et al. Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1548:104-110. |
| APA | Zhao, Liping,Qiao, Juan,Zhang, Ke,Li, Dan,Zhang, Hongyi,&Qi, Li.(2018).Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study.JOURNAL OF CHROMATOGRAPHY A,1548,104-110. |
| MLA | Zhao, Liping,et al."Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study".JOURNAL OF CHROMATOGRAPHY A 1548(2018):104-110. |
入库方式： OAI收割
来源：化学研究所
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