Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing
文献类型：期刊论文
| 作者 | Dong, Qiang1,2,3,4; Li, Xiang1,2,3,4; Wang, Cheng-Zhi3; Xu, Shaohua4; Yuan, Gang4; Shao, Wei4; Liu, Baodong5; Zheng, Yong3; Wang, Hailin; Lei, Xiaoguang4,6,7 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2018-04-24 |
| 卷号 | 115期号:17页码:E4013-E4022 |
| 关键词 | Cse1l Dna Methylation Gene Silencing Nuclear Import Pathway |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1800505115 |
| 英文摘要 | Epigenetic silencing can be mediated by various mechanisms, and many regulators remain to be identified. Here, we report a genome-wide siRNA screening to identify regulators essential for maintaining gene repression of a CMV promoter silenced by DNA methylation. We identified CSE1L (chromosome segregation 1 like) as an essential factor for the silencing of the reporter gene and many endogenous methylated genes. CSE1L depletion did not cause DNA demethylation. On the other hand, the methylated genes derepressed by CSE1L depletion largely overlapped with methylated genes that were also reactivated by treatment with histone deacetylase inhibitors (HDACi). Gene silencing defects observed upon CSE1L depletion were linked to its nuclear import function for certain protein cargos because depletion of other factors involved in the same nuclear import pathway, including KPNAs and KPNB1 proteins, displayed similar derepression profiles at the genome-wide level. Therefore, CSE1L appears to be critical for the nuclear import of certain key repressive proteins. Indeed, NOVA1, HDAC1, HDAC2, and HDAC8, genes known as silencing factors, became delocalized into cytosol upon CSE1L depletion. This study suggests that the cargo specificity of the protein nuclear import system may impact the selectivity of gene silencing. |
| 语种 | 英语 |
| WOS记录号 | WOS:000430697500021 |
| 出版者 | NATL ACAD SCIENCES |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43137]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Zhuqiang; Zhu, Bing |
| 作者单位 | 1.Peking Union Med Coll, Grad Program, Beijing 100730, Peoples R China 2.Chinese Acad Med Sci, Beijing 100730, Peoples R China 3.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China 4.Natl Inst Biol Sci, Beijing 102206, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 6.Peking Univ, Beijing Natl Lab Mol Sci, Dept Biol Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 7.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 8.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Qiang,Li, Xiang,Wang, Cheng-Zhi,et al. Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(17):E4013-E4022. |
| APA | Dong, Qiang.,Li, Xiang.,Wang, Cheng-Zhi.,Xu, Shaohua.,Yuan, Gang.,...&Zhu, Bing.(2018).Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(17),E4013-E4022. |
| MLA | Dong, Qiang,et al."Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.17(2018):E4013-E4022. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。