Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities
文献类型：期刊论文
| 作者 | Wang, Anqi1,3; Feng, Jiesi2,3; Li, Yulong2,3; Zou, Peng1,3 |
| 刊名 | ACS CHEMICAL NEUROSCIENCE
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 9期号:4页码:639-650 |
| ISSN号 | 1948-7193 |
| 关键词 | Biosensors Protein Engineering Fluorescence Imaging Bioluminescence |
| DOI | 10.1021/acschemneuro.7b00455 |
| 英文摘要 | Optical biosensors have been invaluable tools in neuroscience research, as they provide the ability to directly visualize neural activity in real time, with high specificity, and with exceptional spatial and temporal resolution. Notably, a majority of these sensors are based on fluorescent protein scaffolds, which offer the ability to target specific cell types or even subcellular compartments. However, fluorescent proteins are intrinsically bulky tags, often insensitive to the environment, and always require excitation light illumination. To address these limitations, there has been a proliferation of alternative sensor scaffolds developed in recent years, including hybrid sensors that combine the advantages of synthetic fluorophores and genetically encoded protein tags, as well as bioluminescent probes. While still in their early stage of development as compared with fluorescent protein-based sensors, these novel probes have offered complementary solutions to interrogate various aspects of neuronal communication, including transmitter release, changes in membrane potential, and the production of second messengers. In this Review, we discuss these important new developments with a particular focus on design strategies. |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000430642400005 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43309]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Yulong; Zou, Peng |
| 作者单位 | 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Synthet & Funct Biomol Ctr,Minist Educ, Beijing Natl Lab Mol Sci,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100871, Peoples R China 3.Peking Univ, PKU IDG McGovern Inst Brain Res, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Anqi,Feng, Jiesi,Li, Yulong,et al. Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities[J]. ACS CHEMICAL NEUROSCIENCE,2018,9(4):639-650. |
| APA | Wang, Anqi,Feng, Jiesi,Li, Yulong,&Zou, Peng.(2018).Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities.ACS CHEMICAL NEUROSCIENCE,9(4),639-650. |
| MLA | Wang, Anqi,et al."Beyond Fluorescent Proteins: Hybrid and Bioluminescent Indicators for Imaging Neural Activities".ACS CHEMICAL NEUROSCIENCE 9.4(2018):639-650. |
入库方式： OAI收割
来源：化学研究所
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