Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry
文献类型：期刊论文
| 作者 | Liang, Yuan; Zhu, Xiaocui; Zhao, Meiping; Liu, Huwei |
| 刊名 | METHODS
 |
| 出版日期 | 2012-02-01 |
| 卷号 | 56期号:2页码:174-179 |
| 关键词 | Isoprenoid Cytokinins Immunoaffinity Extraction High Performance Liquid Chromatography-quadrupole-time Of Flight Mass Spectrometry Plant Tissue |
| ISSN号 | 1046-2023 |
| DOI | 10.1016/j.ymeth.2011.08.006 |
| 英文摘要 | In this work, we developed a novel class-specific immunoaffinity column for the natural isoprenoid cytokinins (CTKs) by using trans-zeatin riboside as the hapten to generate a complete antigen. By combination with a mixed-mode solid phase extraction step for pre-cleanup and a high performance liquid chromatography-quadrupole-time of flight mass spectrometry for the quantification, an efficient analytical protocol was established which allowed simultaneous quantification of eight endogenous isoprenoid CTKs in Arabidopsis thaliana leaves with a wide linear range from 25 to 500 pg/g fresh weight and a detection limit of 12.5 pg/g fresh weight. The method will be very useful for comprehensive research on the networks of signaling interactions of the active phytohormones and their regulation of the plant functions. (C) 2011 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000302105400009 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43437]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yuan,Zhu, Xiaocui,Zhao, Meiping,et al. Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. METHODS,2012,56(2):174-179. |
| APA | Liang, Yuan,Zhu, Xiaocui,Zhao, Meiping,&Liu, Huwei.(2012).Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry.METHODS,56(2),174-179. |
| MLA | Liang, Yuan,et al."Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry".METHODS 56.2(2012):174-179. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。