Targeted Imaging and Proteomic Analysis of Tumor-Associated Glycans in Living Animals
文献类型：期刊论文
| 作者 | Xie, Ran1,2; Dong, Lu1,2; Huang, Rongbing1,2; Hong, Senlian1,2; Lei, Ruoxing1,2; Chen, Xing1,2 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2014-12-15 |
| 卷号 | 53期号:51页码:14082-14086 |
| 关键词 | Click Chemistry Integrin Ligands Liposomes Sialic Acids Tumor Imaging |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201408442 |
| 英文摘要 | Although it has been well known that dynamic changes in glycosylation are associated with tumor progression, it remains challenging to selectively visualize the cancer glycome in vivo. Herein, a strategy for the targeted imaging of tumor-associated glycans by using ligand-targeted liposomes encapsulating azidosugars is described. The intravenously injected liposomal nanoparticles selectively bound to the cancer-cell-specific receptors and installed azides into the melanoma glycans in a xenograft mouse model in a tissue-specific manner. Subsequently, a copper-free click reaction was performed in vivo to chemoselectively conjugate the azides with a near-infrared fluorescent dye. The glycosylation dynamics during tumor growth were monitored by in vivo fluorescence imaging. Furthermore, the newly synthesized sialylated glycoproteins were enriched during tumor growth and identified by glycoproteomics. Compared with the labeling methods using free azidosugars, this method offers improved labeling efficiency and high specificity and should facilitate the elucidation of the functional role of glycans in cancer biology. |
| 语种 | 英语 |
| WOS记录号 | WOS:000346484400020 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43525]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Xing |
| 作者单位 | 1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn Synthet, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China 2.Peking Univ, Funct Biomol Ctr, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Ran,Dong, Lu,Huang, Rongbing,et al. Targeted Imaging and Proteomic Analysis of Tumor-Associated Glycans in Living Animals[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2014,53(51):14082-14086. |
| APA | Xie, Ran,Dong, Lu,Huang, Rongbing,Hong, Senlian,Lei, Ruoxing,&Chen, Xing.(2014).Targeted Imaging and Proteomic Analysis of Tumor-Associated Glycans in Living Animals.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,53(51),14082-14086. |
| MLA | Xie, Ran,et al."Targeted Imaging and Proteomic Analysis of Tumor-Associated Glycans in Living Animals".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 53.51(2014):14082-14086. |
入库方式： OAI收割
来源：化学研究所
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