Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis
文献类型：期刊论文
| 作者 | Zhang, Yi-Wei; Zhao, Ming-Zhe; Liu, Jing-Xin; Zhou, Ying-Lin; Zhang, Xin-Xiang |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 38期号:3页码:475-482 |
| 关键词 | Capillary Electrophoresis Glycan Analysis Mass Spectrometry Poly(Vinyl Alcohol) |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201401025 |
| 英文摘要 | Glycosylation plays an important role in protein conformations and functions as well as many biological activities. Capillary electrophoresis combined with various detection methods provided remarkable developments for high-sensitivity glycan profiling. The coating of the capillary is needed for highly polar molecules from complex biosamples. A poly(vinyl alcohol)-coated capillary is commonly utilized in the capillary electrophoresis separation of saccharides sample due to the high-hydrophilicity properties. A modified facile coating workflow was carried out to acquire a novel multiple-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable analysis of glycans. The migration time fluctuation was used as index in the optimization of layers and a double layer was finally chosen, considering both the effects and simplicity in fabrication. With migration time relative standard deviation less than 1% and theoretical plates kept stable during 100 consecutive separations, the method was presented to be suitable for the analysis of glycosylation with wide linear dynamic range and good reproducibility. The glycan profiling of enzymatically released N-glycans from human serum was obtained by the presented capillary electrophoresis method combined with mass spectrometry detection with acceptable results. |
| 语种 | 英语 |
| WOS记录号 | WOS:000350175200017 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/43551]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Xin-Xiang |
| 作者单位 | Peking Univ, Coll Chem, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi-Wei,Zhao, Ming-Zhe,Liu, Jing-Xin,et al. Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(3):475-482. |
| APA | Zhang, Yi-Wei,Zhao, Ming-Zhe,Liu, Jing-Xin,Zhou, Ying-Lin,&Zhang, Xin-Xiang.(2015).Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis.JOURNAL OF SEPARATION SCIENCE,38(3),475-482. |
| MLA | Zhang, Yi-Wei,et al."Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis".JOURNAL OF SEPARATION SCIENCE 38.3(2015):475-482. |
入库方式： OAI收割
来源：化学研究所
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