Role of threonine on antifreeze activity of insect antifreeze protein
文献类型：期刊论文
| 作者 | Zhou Yan-Xia; Zhang Yong; Tan Hong-Wei; Jia Zong-Chao; Chen Guang-Ju |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 28期号:3页码:526-529 |
| 关键词 | Insect Antifreeze Protein Mutagenesis Hydrophilicity Hydrophobicity Molecular Dynamics |
| ISSN号 | 0251-0790 |
| 英文摘要 | A series of mutants of the insect antifreeze protein (CfAFP) were designed, their best ice-binding modes were generated by using molecular dynamic simulations, and the interaction energy with ice was computed by semi-empirical molecular orbital methods AM1 and PM3. The results show that the contribution to the CfAFP-ice interaction of the threonine residues depends on the position and number in CfAFP, and Thr residues in TXT-motif play key roles on the recognition and binding of CfAFP and ice. Replacement of thr by other kinds of hydrophilic or hydrophobic amino residues reduced the interaction with ice, resulting in a moderate loss of antifreeze activity. Additionally, based on the structure complement of CfAFP and ice, increasing the size of hydrophobic residues could gain more stable interaction with ice and enhance the antifreeze activity. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245094400031 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/44015]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen Guang-Ju |
| 作者单位 | 1.Beijing Normal Univ, Inst Chem, Beijing 100875, Peoples R China 2.Queens Univ, Dept Biochem, Kingston, ON K7L 3N6, Canada |
| 推荐引用方式 GB/T 7714 | Zhou Yan-Xia,Zhang Yong,Tan Hong-Wei,et al. Role of threonine on antifreeze activity of insect antifreeze protein[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(3):526-529. |
| APA | Zhou Yan-Xia,Zhang Yong,Tan Hong-Wei,Jia Zong-Chao,&Chen Guang-Ju.(2007).Role of threonine on antifreeze activity of insect antifreeze protein.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(3),526-529. |
| MLA | Zhou Yan-Xia,et al."Role of threonine on antifreeze activity of insect antifreeze protein".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.3(2007):526-529. |
入库方式： OAI收割
来源：化学研究所
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