Atomic force microscopic study on topological structures of pBR322 DNA
文献类型：期刊论文
| 作者 | Zhang, PC; Bai, CL; Cheng, YJ; Fang, Y; Wang, ZH; Huang, XT |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 1996-02-01 |
| 卷号 | 39期号:1页码:1-7 |
| 关键词 | Pbr322 Dna Atomic Force Microscopy Topological Structure Image |
| ISSN号 | 1006-9305 |
| 英文摘要 | Plasmid pBR322 DNA (0.5 mg/mL) isolated from Escherichia coli HB101 was suspended in Tris-HCl-EDTA (1 mol/L - 0.1 mol/L, pH 8.5); then a drop of the above solution was deposited on freshly cleaved mica substrate. After adsorption for about 1 min, the sample was stained with phosphotungstic acid. The residual solution was removed with a piece of filter paper. Afterwards the sample was imaged with a home-made atomic force microscope (AFM) in air. The AFM images of pBR322 DNA with a molecular resolution have been obtained. These images show that;pBR322 DNA exists in several different topological structures: (i) relaxed circular DNA with a different diameter; (ii) supercondensed DNA with different particle sizes; (iii) dimeric catenane connected by one relaxed circular molecule and another close-compacted molecule which might be either supercoiled or intramolecular knotted form; (iv) oligomeric catenane with multiple irregular molecules in which DNA is interlocked into a complex oligomer; (v) possibly existing intermediates formed from monomer to oligomer. |
| 语种 | 英语 |
| WOS记录号 | WOS:A1996WA84400001 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/44559]  |
| 专题 | 中国科学院化学研究所 |
| 作者单位 | 1.CHINESE ACAD SCI,INST CHEM,STM LAB,BEIJING 100080,PEOPLES R CHINA 2.NANKAI UNIV,DEPT BIOL,TIANJIN 300071,PEOPLES R CHINA |
| 推荐引用方式 GB/T 7714 | Zhang, PC,Bai, CL,Cheng, YJ,et al. Atomic force microscopic study on topological structures of pBR322 DNA[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1996,39(1):1-7. |
| APA | Zhang, PC,Bai, CL,Cheng, YJ,Fang, Y,Wang, ZH,&Huang, XT.(1996).Atomic force microscopic study on topological structures of pBR322 DNA.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,39(1),1-7. |
| MLA | Zhang, PC,et al."Atomic force microscopic study on topological structures of pBR322 DNA".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 39.1(1996):1-7. |
入库方式： OAI收割
来源：化学研究所
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